An integrated view of gene expression and solute profiles of Arabidopsis tumors:: A genome-wide approach
An integrated view of gene expression and solute profiles of Arabidopsis tumors:: A genome-wide approach
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DOI：
10.1105/tpc.106.044743
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发表时间：
2006-12-01
期刊：
影响因子：
11.6
通讯作者：
Hedrich, Rainer
中科院分区：
文献类型：
--
作者：
Deeken, Rosalia;Engelmann, Julia C.;Hedrich, Rainer
农杆菌T-DNA转化植物细胞是高等植物发育变化的最极端触发因素之一。为了使肿瘤快速生长和发展，需要基因表达谱和代谢适应的特异性变化。增加的运输和代谢通量是生长和肿瘤发展的关键先决条件。应用功能基因组学方法，使用Affytron全基因组微阵列（类似于22，800个基因）来测量基因表达的变化。将拟南芥肿瘤和未感染植物组织的溶质模式与各自的基因表达谱进行比较。阴离子、糖和氨基酸水平的增加与特定酶和溶质转运蛋白基因表达的变化相关。能量代谢关键基因的表达谱，如参与光合作用，线粒体电子传递和发酵的基因，表明肿瘤产生C和N化合物异养和获得能量主要是厌氧。因此，了解植物肿瘤中的基因-代谢物网络有助于确定控制肿瘤发展的机制。
Transformation of plant cells with T-DNA of virulent agrobacteria is one of the most extreme triggers of developmental changes in higher plants. For rapid growth and development of resulting tumors, specific changes in the gene expression profile and metabolic adaptations are required. Increased transport and metabolic fluxes are critical preconditions for growth and tumor development. A functional genomics approach, using the Affymetrix whole genome microarray (similar to 22,800 genes), was applied to measure changes in gene expression. The solute pattern of Arabidopsis thaliana tumors and uninfected plant tissues was compared with the respective gene expression profile. Increased levels of anions, sugars, and amino acids were correlated with changes in the gene expression of specific enzymes and solute transporters. The expression profile of genes pivotal for energy metabolism, such as those involved in photosynthesis, mitochondrial electron transport, and fermentation, suggested that tumors produce C and N compounds heterotrophically and gain energy mainly anaerobically. Thus, understanding of gene-to-metabolite networks in plant tumors promotes the identification of mechanisms that control tumor development.