Carrier Status of Leptospirosis Among Cattle in Sri Lanka: A Zoonotic Threat to Public Health
Carrier Status of Leptospirosis Among Cattle in Sri Lanka: A Zoonotic Threat to Public Health
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DOI：
10.1111/tbed.12014
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发表时间：
2014-02-01
影响因子：
4.3
通讯作者：
Tamashiro, H.
中科院分区：
文献类型：
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作者：
Gamage, C. D.;Koizumi, N.;Tamashiro, H.
钩端螺旋体病是一种具有全球重要性的人畜共患疾病，也是斯里兰卡的一种应报告疾病。最近对人类钩端螺旋体病的研究表明，牛可能是该国人类感染的重要宿主之一。然而，当地缺乏关于牛钩端螺旋体病的信息，包括其对人类传播的影响。因此，本研究试图确定斯里兰卡牛中致病性钩端螺旋体属的携带状态。在市兽医进行的常规检查程序期间，从科隆博市的肉类检查大厅共采集了164份牛肾脏样本。提取DNA，采用巢式PCR检测钩体flaB基因。对扩增子进行测序，并计算同源距离。在164份样本中，20份（12.2%）为flaB-PCR阳性。结果表明，钩端螺旋体属的3个种分别为伯氏钩端螺旋体（10/20，50%）、克氏钩端螺旋体（7/20，35%）和问号钩端螺旋体（3/20，15%）。结果表明，高比例的抽样牛窝藏各种致病性钩端螺旋体属，这可以作为人类疾病的重要水库。
Leptospirosis is a zoonotic disease of global importance and one of the notifiable diseases in Sri Lanka. Recent studies on human leptospirosis have suggested that the cattle could be one of the important reservoirs for human infection in the country. However, there is a dearth of local information on bovine leptospirosis, including its implications for human transmission. Thus, this study attempted to determine the carrier status of pathogenic Leptospira spp in cattle in Sri Lanka. A total of 164 cattle kidney samples were collected from the meat inspection hall in Colombo city during routine inspection procedures conducted by the municipal veterinary surgeons. The DNA was extracted and subjected to nested PCR for the detection of leptospiral flaB gene. Amplicons were sequenced, and phylogenic distances were calculated. Of 164 samples, 20 (12.2%) were positive for flaB-PCR. Sequenced amplicons revealed that Leptospira species were deduced to L.borgpetersenii (10/20, 50%), L.kirschneri (7/20, 35%) and L.interrogans (3/20, 15%). The results indicate that a high proportion of the sampled cattle harbour a variety of pathogenic Leptospira spp, which can serve as important reservoirs for human disease.