Immunohistochemical and quantitative competitive PCR analyses of midkine and pleiotrophin expression in cervical cancer
Immunohistochemical and quantitative competitive PCR analyses of midkine and pleiotrophin expression in cervical cancer
复制标题
DOI：
10.1016/s0090-8258(02)00070-7
复制
发表时间：
2003-03-01
影响因子：
4.7
通讯作者：
Woo, BH
中科院分区：
文献类型：
--
作者：
Moon, HS;Park, WI;Woo, BH
Objective.本研究的目的是确定中期因子（MK）和多效生长因子（PTN）在宫颈癌中的表达。三级教学医院前瞻性研究。正常和癌性宫颈组织获自健康女性（n=19）和宫颈癌患者（n=42）。采用定量竞争PCR和免疫组织化学方法检测MK和PTN mRNA和蛋白的表达。MK和PTN mRNA和蛋白表达与肿瘤分期和大小有关。中期因子和多效生长因子mRNA在宫颈癌组织中的表达均高于正常宫颈组织（MK分别为175.59 ± 63.3fmol和1.00 ± 0.18fmol; PTN分别为3.18 ± 1.25fmol和0.86 ± 0.12fmol，P
Objective. The aim of this study was to determine midkine (MK) and pleiotrophin (PTN) expression in cervical cancer.Methods. Prospective study in tertiary teaching hospital. Normal and cancerous cervical tissues were obtained from healthy women (n=19) and from patients with cervical cancer (n=42). The expressions of MK and PTN mRNA and protein were examined by quantitative competitive PCR and by immunohistochemistry. MK and PTN mRNA and protein expressions were examined with respect to tumor stage and size.Results. The expressions of midkine and pleiotrophin mRNA in cervical cancer were higher than those in the normal cervix (MK, 175.59+/-63.3 vs 1.00+/-0.18 fmol, respectively; PTN, 3.18+/-1.25 vs. 0.86+/-0.12 fmol, respectively, P