Stable isotope signatures and trophic-step fractionation factors of fish tissues collected as non-lethal surrogates of dorsal muscle
Stable isotope signatures and trophic-step fractionation factors of fish tissues collected as non-lethal surrogates of dorsal muscle
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DOI：
10.1002/rcm.7247
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发表时间：
2015-08-30
影响因子：
2
通讯作者：
Britton, J. Robert
中科院分区：
文献类型：
--
作者：
Busst, Georgina M. A.;Basic, Tea;Britton, J. Robert
原理：背白色肌肉是用稳定同位素分析分析鱼类营养研究的标准组织。由于肌肉通常是破坏性的收集，鳍组织和鳞片经常被用作非致命的替代品；我们研究了鳞片和鳍组织作为肌肉替代品的效用。方法：用元素分析仪和同位素比值质谱仪对10种鲤科鱼类的肌肉、鳍和鳞的δ N-15和δ C-13进行测定。这些鱼包括：(1)来自野外的样本，(2)来自水族箱的样本，使用6个物种，饲养120天，喂食单一食物资源。研究了每个物种和整个数据集的肌肉、鳍和鳞片同位素比率之间的关系，并测试了从鳍和鳞片值预测肌肉同位素比率的四种方法的有效性。然后计算实验鱼的三种组织与其食物资源之间的分异因子，并将其应用于贝叶斯混合模型，以评估它们对鱼的饮食预测的影响。结果：每个物种的三种组织的同位素数据是不同的，但显着相关，可以从两个替代品估计肌肉值。物种特异性方程对鳞和鳍同位素比值的修正误差最小（误差< 0.6%）。N-15值的分馏系数在其他树种的分馏系数范围内，但C-13值的分馏系数往往更高。他们对混合模型中两种鱼类种群的数据的应用导致了饮食预测的重大变化。结论：在食物网研究中，鳞片和鳍组织是背肌的有力替代品，因为当使用特定物种的方法时，它们可以在可接受的误差范围内提供肌肉值的估计。他们得出的分馏因子也可以应用于从δ N-15和δ C-13值预测鱼类饲料组成的模型。版权所有：John Wiley & Sons， Ltd
RATIONALE: Dorsal white muscle is the standard tissue analysed in fish trophic studies using stable isotope analyses. As muscle is usually collected destructively, fin tissues and scales are often used as non-lethal surrogates; we examined the utility of scales and fin tissue as muscle surrogates.METHODS: The muscle, fin and scale delta N-15 and delta C-13 values from 10 cyprinid fish species determined with an elemental analyser coupled with an isotope ratio mass spectrometer were compared. The fish comprised (1) samples from the wild, and (2) samples from tank aquaria, using six species held for 120 days and fed a single food resource. Relationships between muscle, fin and scale isotope ratios were examined for each species and for the entire dataset, with the efficacy of four methods of predicting muscle isotope ratios from fin and scale values being tested. The fractionation factors between the three tissues of the laboratory fishes and their food resource were then calculated and applied to Bayesian mixing models to assess their effect on fish diet predictions.RESULTS: The isotopic data of the three tissues per species were distinct, but were significantly related, enabling estimations of muscle values from the two surrogates. Species-specific equations provided the least erroneous corrections of scale and fin isotope ratios (errors < 0.6%). The fractionation factors for delta N-15 values were in the range obtained for other species, but were often higher for delta C-13 values. Their application to data from two fish populations in the mixing models resulted in significant alterations in diet predictions.CONCLUSIONS: Scales and fin tissue are strong surrogates of dorsal muscle in food web studies as they can provide estimates of muscle values within an acceptable level of error when species-specific methods are used. Their derived fractionation factors can also be applied to models predicting fish diet composition from delta N-15 and delta C-13 values. Copyright (C) 2015 John Wiley & Sons, Ltd.