VILLIN - A CYTOSKELETAL PROTEIN AND A DIFFERENTIATION MARKER EXPRESSED IN SOME HUMAN ADENOCARCINOMAS
VILLIN - A CYTOSKELETAL PROTEIN AND A DIFFERENTIATION MARKER EXPRESSED IN SOME HUMAN ADENOCARCINOMAS
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DOI：
10.1007/bf02899208
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发表时间：
1987-01-01
期刊：
影响因子：
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通讯作者：
LOUVARD, D
中科院分区：
文献类型：
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作者：
MOLL, R;ROBINE, S;LOUVARD, D
我们通过对冷冻切片应用免疫荧光显微镜检查，并使用兔抗血清和绒毛特异性单克隆抗体对组织提取物进行免疫印迹方法，研究了绒毛蛋白（一种刷状缘微绒毛典型的微丝相关肌动蛋白结合蛋白）在多种人类癌症中的表达。所测试的所有 24 例原发性和转移性结直肠腺癌均呈绒毛一致强阳性，免疫细胞化学标记集中在管腔细胞边缘。在分化差的肿瘤区域中，残基小管被染色。研究的所有六种胃管状腺癌以及两种胆囊腺癌和肝细胞癌也均为绒毛阳性。在 14 个胰腺腺癌中，有 12 个可检测到绒毛蛋白；在其中一些情况下，其分布是异质的。在研究的 21 例肾细胞癌中，13 例透明细胞肿瘤（尤其是 II 级）中的 9 例以及所有 4 例嗜铬细胞肿瘤中均检测到绒毛蛋白阳性；然而，所研究的所有四种嫌色细胞肿瘤均为阴性。 11 个子宫内膜癌中有 4 个，但 9 个卵巢癌均未（均匀或局部）绒毛阳性。在研究的 18 例肺腺癌中，1 例绒毛蛋白一致阳性，4 例局灶性阳性，其余均为阴性。研究的所有其他上皮肿瘤，包括 12 例乳腺癌和 7 例上皮性或双相性胸膜间皮瘤，绒毛阴性。我们的结果表明，即使有组织的刷状缘结构已经丢失，肠上皮细胞中绒毛蛋白的表达在其相应的癌中始终保持不变。一些子宫内膜和肺腺癌中存在绒毛蛋白，与相应的正常上皮细胞中不存在绒毛蛋白相反，表明该蛋白在增生、不典型增生或癌变过程中新表达。确定转移性腺癌中绒毛蛋白的存在与否及其免疫细胞化学染色模式可能对确定原发肿瘤的类型和部位有一定帮助。
We studied the expression of villin, a microfilament-associated, actin-binding protein typical of brush-border microvilli, in a variety of human carcinomas by applying immunofluorescence microscopy to frozen sections and immunoblotting methods to tissue extracts using a rabbit antiserum and a monoclonal antibody specific for villin. All of the 24 primary and metastatic colorectal adenocarcinomas tested were uniformly and strongly positive for villin, with the immunocytochemical labeling concentrated at the luminal cell margin. In poorly differentiated tumor areas, rudimentary tubules were stained. All of the six tubular adenocarcinomas of the stomach studied as well as two adenocarcinomas of the gall bladder and hepatocellular carcinoma were also villin-positive. Villin was detectable in 12 of 14 adenocarcinomas of the pancreas; in some of these cases, its distribution was heterogeneous. Among 21 renal cell carcinomas investigated, positivity for villin was seen in nine of 13 clear cell tumors (especially those of grade II), and in all four chromophilic cell tumors; however, all four chromophobe cell tumors studied were negative. Four of 11 endometrial, but none of nine ovarian carcinomas were (uniformly or focally) villin positive. Of 18 adenocarcinomas of the lung studied, one was uniformly and four focally positive for villin, while the remainder were negative. All of the other epithelial tumors studied, including 12 adenocarcinomas of the breast and seven epithelial or biphasic pleural mesotheliomas, were villin negative. Our results show that the expression of villin in intestinal epithelial cells is consistently maintained in their corresponding carcinomas, even when the organized brush-border structure has been lost. The presence of villin in some endometrial and pulmonary adenocarcinomas-in constrst to its absence in the respective normal epithelial-suggests that this protein is newly expressed during hyperplasia, dysplasia, or carcinogenesis. Determining the presence or absence of villin and its immunocytochemical staining pattern in metastatic adenocarcinomas may be of some help in determining the type and site of the primary tumor.