Estimating glucose metabolism using glucose analogs and two tracer kinetic models in isolated rabbit heart.
Estimating glucose metabolism using glucose analogs and two tracer kinetic models in isolated rabbit heart.
复制标题
使用葡萄糖类似物和离体兔心脏中的两种示踪动力学模型估计葡萄糖代谢。
DOI：
10.1152/ajpheart.1998.275.2.h668
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Budinger,TF
中科院分区：
文献类型：
--
作者：
Marshall,RC;Powers-Risius,P;Huesman,RH;Reutter,BW;Taylor,SE;Maurer,HE;Huesman,MK;Budinger,TF
The purpose of this investigation was to1) evaluate the relative accuracy of the Sokoloff and Patlak tracer kinetic models in estimating glucose metabolic rate (GMR) in the presence and absence of insulin;2) evaluate the effect of nutritional state on the lumped constant (LC); and3) compare the kinetics of 2-fluoro-2-deoxy-d-[14C]glucose (FDG) and 2-deoxy-d-[3H]glucose (DG) membrane transport and phosphorylation. The experimental preparation was the isolated, red blood cell-albumin-perfused rabbit heart. Our results showed that both tracer kinetic models provided GMR estimates that correlated well with the Fick method (for FDG,R= 0.84 and 0.91 for the Sokoloff and Patlak models, respectively); nutritional state did not affect the LC; and FDG and DG have different transport and/or phosphorylation parameters. We also observed that1) the addition of a fourth compartment to the Sokoloff model reduced the mean squared error between measured and modeled data by a factor of 7.4;2) a longer time (21.8 min) was required to obtain a linear phase of the Patlak plot than is allowed in clinical studies; and3) accurate GMR estimates were obtained only by using different LCs reflecting insulin’s presence or absence. Our results indicate potential sources of error in the use of FDG and positron emission tomography to quantify GMR in patients.
