Purification and identification of novel antioxidant peptides from egg white protein and their antioxidant activities
Purification and identification of novel antioxidant peptides from egg white protein and their antioxidant activities
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DOI：
10.1016/j.foodchem.2014.11.142
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发表时间：
2015-05-15
期刊：
影响因子：
8.8
通讯作者：
Chen, Feng
中科院分区：
文献类型：
--
作者：
Liu, Jingbo;Jin, Yan;Chen, Feng
本研究的目的是从蛋清蛋白水解物(EWPH)中分离抗氧化肽，并通过LC串联质谱学和中红外光谱(MIR)鉴定新的抗氧化肽。用氨基酸分析仪分析了多肽的氨基酸组成。其中3个新多肽的相对分子质量分别为628.64 Da、630.71 Da和684.1 Da，分别为Asp-His-Thr-Lys-Glu(DHTKE)、Phe-Phe-Glu-Phe-His(FFGFN)和Met-Pro-Asp-Ala-His-Leu(MPDAHL)。DHTKE对氧自由基的吸收能力最强(P<；0.05)。FFGFN和MPDAHL清除50%DPPH自由基的浓度分别为80 mM和60 mM。因此，这三种多肽在功能食品方面有潜在的应用前景，也可以作为营养食品化合物。(C)2014爱思唯尔有限公司。保留所有权利。
The aim of this study was to isolate antioxidant peptides from egg white protein hydrolysates (EWPH) and identify novel antioxidant peptides by LC tandem mass spectrometric and mid-infrared spectroscopy (MIR). The amino acid composition of peptides was also analyzed by amino acid analyzer on the basis of ninhydrin reaction. Three novel peptides with molecular weights of 628.64 Da, 630.71 Da, and 684.1 Da were identified as Asp-His-Thr-Lys-Glu (DHTKE), Phe-Phe-Glu-Phe-His (FFGFN) and Met-Pro-Asp-Ala-His-Leu (MPDAHL), respectively. DHTKE exhibited the best oxygen radical absorbance capacity (P< 0.05). The concentration of FFGFN and MPDAHL to scavenge 50% of DPPH radicals was 80 mM and 60 mM, respectively. Thus, the three peptides may have potential applications as a functional food, which could also be used as nutraceutical compounds. (c) 2014 Elsevier Ltd. All rights reserved.