Bio/abiotic interface constructed from nanoscale DNA dendrimer and conducting polymer for ultrasensitive biomolecular diagnosis.
Bio/abiotic interface constructed from nanoscale DNA dendrimer and conducting polymer for ultrasensitive biomolecular diagnosis.
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由纳米级DNA树枝状聚合物和用于超敏化生物分子诊断的聚合物构建的生物/非生物界面。
DOI：
10.1002/smll.200900369
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发表时间：
2009-08-03
期刊：
影响因子：
13.3
通讯作者：
Ho, Chih-Ming
中科院分区：
文献类型：
--
作者：
Wei, Fang;Liao, Wei;Xu, Zheng;Yang, Yang;Wong, David T.;Ho, Chih-Ming
对于检测固定化生物标志物的传感器，表面和流体介质之间的界面在确定电化学检测过程中的信号和噪声水平方面起着重要作用。当蛋白质直接固定在金属电极上时，通过表面-蛋白质相互作用的蛋白质变性导致低活性和低信号水平。基于导电聚合物的界面可以防止蛋白质构象变化并缓解这一问题。我们将DNA树枝状聚合物引入传感器表面的界面膜中，以进一步提高传感器的性能。DNA树枝状聚合物是由短的DNA序列构成的纳米级树枝状聚合物，它可以很容易地嵌入到非生物导电聚合物基质中，并且对大多数生物物种具有生物相容性。在这项工作中，DNA-树枝状聚合物和聚吡咯（DDPpy）形成电化学传感器上的生物-非生物界面。使用两种唾液蛋白标志物（IL-8和IL-1β）和一种mRNA唾液标志物（IL-8）的检测来证明DDPpy传感器的效率。蛋白质的检测限（LOD）达到100-200 fg/ml，比没有DNA-树状聚合物界面时提高了三个数量级。确定IL-8 mRNA的LOD为10 μ M。用于检测的典型样品体积为4 μl，因此LOD仅达到25个目标分子（40 yocto mole）。
For sensors detecting immobilized biomarkers, the interface between the surface and the fluid medium plays an important role in determining the levels of signal and noise in the electrochemical detection process. When protein is directly immobilized on the metal electrode, denaturation of the protein by surface-protein interaction results in low activity and low signal level. The conducting polymer based interface can prevent the protein conformation change and alleviate this problem. We introduce the DNA-dendrimer into the interfacial film on the sensor surface to further improve the sensor performance. DNA-dendrimer is a nano-scale dendrite constructed of short DNA sequences, which can be easily incorporated into the abiotic conducting polymer matrix and is biocompatible to most bio-species. In this work, DNA-dendrimer and polypyrrole (DDPpy) form the bio-abiotic interface on electrochemical sensors. Detections of two salivary protein markers (IL-8 and IL-1β) and one mRNA salivary marker (IL-8) were used to demonstrate the efficiency of the DDPpy sensor. The limit of detection (LOD) of protein has achieved 100-200 fg/ml, which are three orders of magnitude better than that without the DNA-dendrimer interface. An LOD of 10 aM was established for IL-8 mRNA. The typical sample volume used in the detection is 4 μl, thus the LOD reaches only 25 target molecules (40 yocto mole).
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影响因子：
4.1
作者：
Chung, J. W.;Park, J. M.;Pyun, J. C.
通讯作者：
Pyun, J. C.
影响因子：
7.4
作者：
Lee, Chi-Ying;Nguyen, Phuong-Cac T.;Castner, David G.
通讯作者：
Castner, David G.
影响因子：
3.9
作者：
Boozer, C;Chen, SF;Jiang, SY
通讯作者：
Jiang, SY
影响因子：
4.9
作者：
Calonder, C;Matthew, HWT;Van Tassel, PR
通讯作者：
Van Tassel, PR
DOI：
10.1073/pnas.1132025100
发表时间：
2003-05-27
影响因子：
11.1
作者：
Fan, CH;Wang, S;Heeger, AJ
通讯作者：
Heeger, AJ