Farnesol induces apoptosis and oxidative stress in the fungal pathogen Penicillium expansum
Farnesol induces apoptosis and oxidative stress in the fungal pathogen Penicillium expansum
复制标题
金合欢醇诱导真菌病原体扩展青霉的细胞凋亡和氧化应激
DOI：
10.3852/09-176
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发表时间：
2010-03-01
期刊：
影响因子：
2.8
通讯作者：
Cheng, Yunjiang
中科院分区：
文献类型：
--
作者：
Liu, Pu;Luo, Li;Cheng, Yunjiang
本研究旨在评估金合欢醇 (FOH) 对 P. expansum 生长的影响。通过在每次 FOH 处理后计数菌落形成单位 (CFU) 来确定 P. expansum 细胞的活力。通过使用 Hoechst 33258、TUNEL（末端脱氧核苷酸转移酶荧光素-12-dUTP 缺口末端标记）、Annexin-V FITC 和氧化剂敏感探针 H2DCFDA（二氯二氢荧光素二乙酸盐）染色来分析 FOH 处理的真菌细胞的形态变化。 FOH强烈抑制菌丝的生长。菌丝细胞显示出细胞凋亡的标志，包括染色质浓缩、DNA 断裂、磷脂酰丝氨酸 (PS) 外化、半胱天冬酶激活、细胞内活性氧 (ROS) 产生，但没有核小体梯子产生。透射电子显微镜（TEM）观察到的异常细胞超微结构表明，细胞超微结构（尤其是线粒体）的解体与FOH诱导的细胞死亡有关。综上所述，我们证明 FOH 抑制 P. expansum 的生长，并通过激活后半胱天冬酶、产生 ROS 和分解细胞超微结构来促进细胞凋亡。
This study was conducted to evaluate the effect of farnesol (FOH) on the growth of P. expansum. The viability of P. expansum cells was determined by counting the colony forming units (CFU) after each FOH treatment. Morphological changes of FOH-treated fungal cells were analyzed by staining with Hoechst 33258, TUNEL (terminal deoxynucleotidyl transferase fluorescein-12-dUTP nick end labeling), Annexin-V FITC and the oxidant-sensitive probe H2DCFDA (dichlorodihydrofluorescein diacetate). FOH strongly inhibited the growth of hyphae. The hyphal cells showed the hallmarks of apoptosis including chromatin condensation, DNA fragmentation, phosphatidylserine (PS) externalization, caspases activation, intracellular reactive oxygen species (ROS) generation but without nucleosomal ladder production. The abnormal cellular ultrastructure observed by transmission electron microscope (TEM) indicated that disintegration of cellular ultrastructure (especially for mitochondria) was linked to FOH-induced cell death. Taken together we demonstrated that FOH inhibits the growth of P. expansum and promotes apoptosis via activation of metacaspases, production of ROS and disintegration of cellular ultrastructure.