Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG
Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG
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DOI：
10.1016/j.febslet.2012.07.065
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发表时间：
2012-09-21
期刊：
影响因子：
3.5
通讯作者：
Tachibana, Hirofumi
中科院分区：
文献类型：
--
作者：
Tsukamoto, Shuntaro;Yamashita, Shuya;Tachibana, Hirofumi
（-）-表没食子儿茶素-3-O-没食子酸酯（EGCG）表现出通过67-kDa层粘连蛋白受体（67 LR）介导的抗肿瘤活性。在这项研究中，我们发现暴露于低O-2水平（5%）会降低67 LR蛋白水平，而不会影响HIF-1 α的表达。我们还发现，在各种癌细胞中，EGCG诱导的抗癌活性在低O-2水平（5%）下被废除。值得注意的是，用蛋白酶体抑制剂治疗，防止了67 LR的下调，并在5%O-2下恢复了对EGCG的敏感性。综上所述，67 LR的表达对O-2分压高度敏感，并且在癌细胞中EGCG的活性可以通过O-2分压来调节。(C)2012年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O-2 levels (5%), without affecting the expression of HIF-1 alpha. We also found that EGCG-induced anticancer activity is abrogated under low O-2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O-2. In summary, 67LR expression is highly sensitive to O-2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O-2 partial pressure. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.