The effect of time and origin of harvest on the in vitro biological activity of Palmaria palmata protein hydrolysates
The effect of time and origin of harvest on the in vitro biological activity of Palmaria palmata protein hydrolysates
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DOI：
10.1016/j.foodres.2014.04.035
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发表时间：
2014-08-01
影响因子：
8.1
通讯作者：
FitzGerald, Richard J.
中科院分区：
文献类型：
--
作者：
Harnedy, Padraigin A.;Soler-Vila, Anna;FitzGerald, Richard J.
研究了起始蛋白组成、来源和收获时间对掌叶蛋白水解物体外酪氨酸酶、二肽基肽酶（DPP）IV和血管紧张素转换酶（ACE）抑制活性和抗氧化活性的影响。电泳图谱显示，在一年中的不同时间收获的红色macrophages Palmaria palmata水蛋白提取物显着差异。从野生和养殖的Palmaria palmata样品中提取的水溶性蛋白质谱没有显著差异。用Alcalase 2.4 Land Corolase PP酶解4月、7月和10月从野生植物中收获的Palmaria palmata样品和4月收获的延绳钓栽培样品的蛋白提取物。当以10 mg/ml测试时，显示水解产物抑制酪氨酸酶37- 56%。水解产物的氧自由基吸收能力（ORAC）和铁还原抗氧化能力（FRAP）值分别为323-494和8.9-19.9 μ mol trolox当量/克。掌叶藻水解物对DPP-IV（IC_（50）：1.60-4.24 mg/mL）和ACE（IC_（50）：0.14 ~ 035 mg/mL）活性均有抑制作用。起始蛋白组分对酪氨酸酶抑制活性有显著影响，而蛋白组分和水解酶制剂对DPP-IV抑制活性和抗氧化活性有显著影响。一般来说，样品的来源，野生或栽培，对蛋白质水解产物的体外生物活性没有影响。结果表明，棕榈叶水解物具有抗氧化和抑制酪氨酸酶的作用，可作为保健成分和食品防腐剂。(C)2014爱思唯尔有限公司版权所有。
The effect of starting protein composition, and the origin and time of harvesting on the in vitro tyrosinase, dipeptidyl peptidase (DPP) IV and angiotensin converting enzyme (ACE) inhibitory and antioxidant activity of Palmaria palmata protein hydrolysates were investigated. Electrophoretic profiles showed significant differences in aqueous protein extracts obtained from the red macroalga Palmaria palmata harvested at different times of the year. No significant difference was observed in aqueous protein profiles extracted from wild and aquacultured samples of Palmaria palmata. Protein extracts from Palmaria palmata samples harvested from wild plants in April, July and October, and samples cultivated on longlines and harvested in April were hydrolysed with Alcalase 2.4 Land Corolase PP. The hydrolysates, when tested at 10 mg/ml, were shown to inhibit tyrosinase by 37-56%. The oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) values of the hydrolysates ranged from 323-494 and 8.9-19.9 mu mol trolox equivalents per gram, respectively. The Palmaria palmata hydrolysates inhibited DPP-IV (IC50: 1.60-4.24 mg/mL) and ACE (IC50: 0.14-035 mg/mL) activities. The starting protein composition had a significant effect on the tyrosinase inhibitory activity, while protein composition and hydrolytic enzyme preparation had a significant effect on DPP-IV inhibitory and antioxidant activities. In general, the origin of the samples, wild or cultivated, had no effect on the in vitro biological activity of the protein hydrolysates. The results show that Palmaria palmata hydrolysates may have potential applications as health enhancing ingredients and as food preservatives due to their antioxidant and tyrosinase inhibitory effects. (C) 2014 Elsevier Ltd. All rights reserved.