An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding.
An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding.
复制标题
分析胰腺癌胰周脂肪侵袭的体外三维器官模型：基于胶原凝胶嵌入的培养系统。
DOI：
10.1007/978-1-4939-8879-2_11
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Nakamura M.
中科院分区：
文献类型：
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作者：
Okumura T;Ohuchida K;Nakamura M.
三维培养系统比传统的二维培养更能反映生物环境。此外，三维培养是一种强有力的实验工具，可以在体外分析癌细胞和基质细胞之间的直接相互作用。在此，我们描述了一种器官型脂肪侵袭模型的方案，该模型是一种模拟胰腺癌胰腺外侵袭（PDAC）的新型培养系统。这种新的模型是基于胶原蛋白I凝胶包埋方法，使我们能够分析癌细胞和脂肪组织之间的功能和组织学相互作用。
Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue.