Endothelial-Specific Cre Mouse Models.
Endothelial-Specific Cre Mouse Models.
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DOI：
10.1161/atvbaha.118.309669
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
Neal A
中科院分区：
文献类型：
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作者：
Payne S;De Val S;Neal A
血管生物学领域已经从使用Cre和Cre/ERT 2小鼠模型在时间和空间上操纵内皮内的基因表达中获得了巨大的洞察力。模型可用于组成性地或诱导性地调节所有内皮细胞（EC）或特定子集中的基因表达。然而，在选择等位基因和分析所得表型时应谨慎：许多名称相似的Cre模型具有不同的活性模式，而异位或不一致的Cre或Cre/ERT 2表达可能会显著影响结果。为了消除以前的数据，并提供一个资源，以帮助适当的实验设计，在这里，我们总结了什么是已知的Cre重组酶活性在最广泛使用的内皮特异性Cre和Cre/ERT 2小鼠模型。
The field of vascular biology has gained enormous insight from the use of Cre and Cre/ERT2 mouse models to temporally and spatially manipulate gene expression within the endothelium. Models are available to constitutively or inducibly modulate gene expression in all, or a specified subset of endothelial cells (ECs). However, caution should be applied to both the selection of allele and the analysis of resultant phenotype: many similarly-named Cre models have divergent activity patterns, while ectopic or inconsistent Cre or Cre/ERT2 expression can dramatically affect results. In an effort to disambiguate previous data and to provide a resource to aid appropriate experimental design, here we summarise what is known about Cre recombinase activity in the most widely used endothelial-specific Cre and Cre/ERT2 mouse models.