Oroxylin a could be a Promising Radiosensitizer for Esophageal Squamous Cell Carcinoma by Inducing G2/M Arrest and Activating Apoptosis
Oroxylin a could be a Promising Radiosensitizer for Esophageal Squamous Cell Carcinoma by Inducing G2/M Arrest and Activating Apoptosis
复制标题
DOI：
10.1007/s12253-016-0106-1
复制
发表时间：
2017-04-01
影响因子：
2.8
通讯作者：
Cai, Jing
中科院分区：
文献类型：
--
作者：
Tan, Cheng;Qian, Xia;Cai, Jing
目的探讨Oroxylin A对食管癌细胞的放射增敏作用及Oroxylin A与放射治疗的最佳方案。通过CCK 8测定法估计细胞增殖。通过克隆形成存活试验评价放射增敏作用。流式细胞仪检测细胞凋亡及细胞周期的变化。Western blot分析Survivin和细胞周期调控因子的表达。在有或没有Oroxylin A的情况下，发现响应于辐射的剂量依赖性细胞存活减少。细胞凋亡率呈剂量依赖性增高。此外，Oroxylin A还可使食管鳞癌细胞阻滞于G2/M期，并通过调节cyclin B1和Cdc 2蛋白的表达而增强其放射增敏作用。Oroxylin A可能通过诱导G2/M期阻滞和激活细胞凋亡而成为食管鳞癌放射增敏剂。
To evaluate the radiosensitization of Oroxylin A on esophageal carcinoma cell as well as the optimal scheduling of Oroxylin A and radiotherapy (RT). Cell proliferation was estimated by a CCK8 assay. Radiosensitization was evaluated by a clonogenic survival assay. The progressions of Cell apoptosis and Cell cycle were investigated by flow cytometry. Expressions of survivin and cell cycle regulators were evaluated by Western blot analysis. A dose-dependent cell survival reduction was found in response to radiation with or without Oroxylin A. The apoptosis rates were remarkably dose-dependent higher in combination groups than in either Oroxylin A or radiation alone group. Besides, Oroxylin A could obviously radiosensitize ESCC cells by arresting tumor cells in G2/M phase and regulating cyclin B1 and Cdc 2 protein expression. Oroxylin A could be a promising radiosensitizer for esophageal squamous cell carcinoma by inducing G2/M phase blocking and activating cell apoptosis.