Mapping QTLs for phosphorus deficiency tolerance in rice (Oryza sativa L.)
Mapping QTLs for phosphorus deficiency tolerance in rice (Oryza sativa L.)
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DOI：
10.1007/s001220051030
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发表时间：
1998-12-01
影响因子：
5.4
通讯作者：
Huang, N
中科院分区：
文献类型：
--
作者：
Ni, JJ;Wu, P;Huang, N
扩增片段长度多态性（AFLP）技术与选择性基因分型相结合，用于绘制与水稻磷（P）缺乏耐受性相关的数量性状位点（QTL）。将耐缺磷品种 IR20 与 IR55178-3B-9-3（对缺磷敏感）杂交，通过单种子血统产生 285 个重组自交系 (RIL)。通过在 P 充足 (10.0 mg/l) 和 P 缺乏 (0.5 mg/l) 营养液中培养 RIL，并在播种后 28 天测定其相对分蘖能力，以及在播种后 42 天测定相对芽干重和相对根干重，对 RIL 的性状进行表型分析。每种极端 RIL（敏感和耐受）中的 42 名及其父母均接受了 AFLP 分析。构建了由 217 个 AFLP 标记组成的图谱。其长度为1371.8 cM，平均间隔尺寸为7.62 cM。为了将连锁群分配给染色体，分布在12条染色体上的30个AFLP和26个RFLP标记被用作锚定标记。基于构建的图谱，P-缺乏耐受性的主要QTL（命名为PHO）位于12号染色体上，并由同一染色体上的RFLP标记RG9和RG241确认。几个次要 QTL 被定位在 1、6 和 9 号染色体上。
The amplified fragment length polymorphism (AFLP) technique combined with selective genotyping was used to map quantitative trait loci (QTLs) associated with tolerance for phosphorus (P) deficiency in rice. P deficiency tolerant cultivar IR20 was crossed to IR55178-3B-9-3 (sensitive to P-deficiency) and 285 recombinant inbred lines (RILs) were produced by single-seed descent. The RILs were phenotyped for the trait by growing them in P-sufficient (10.0 mg/l) and P-deficient (0.5 mg/l) nutrient solution and determining their relative tillering ability at 28 days after seeding, and relative shoot dry weight and relative root dry weight at 42 days after seeding. Forty two of each of the extreme RILs (sensitive and tolerant) and the parents were subjected to AFLP analysis. A map consisting of 217 AFLP markers was constructed. Its length was 1371.8 cM with an average interval size of 7.62 cM. To assign linkage groups to chromosomes, 30 AFLP and 26 RFLP markers distributed over the 12 chromosomes were employed as anchor markers. Based on the constructed map, a major QTL for P-deficiency tolerance, designated PHO, was located on chromosome 12 and confirmed by RFLP markers RG9 and RG241 on the same chromosome. Several minor QTLs were mapped on chromosomes 1, 6, and 9.