Characterization of Drosophila melanogaster cytochrome P450 genes
Characterization of Drosophila melanogaster cytochrome P450 genes
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DOI：
10.1073/pnas.0812141106
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发表时间：
2009-04-07
影响因子：
11.1
通讯作者：
Daborn, Phillip J.
中科院分区：
文献类型：
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作者：
Chung, Henry;Sztal, Tamar;Daborn, Phillip J.
细胞色素p450形成了一个庞大而多样的含血红素蛋白家族，能够进行许多不同的酶促反应。在哺乳动物和植物中，已知一些p450参与激素合成等过程的必要反应，而其他p450参与环境化合物的解毒。一般来说，人们对昆虫p450的功能了解较少。我们研究了黑腹果蝇P450在胚胎和2个3龄幼虫中的表达模式。我们发现许多p450表达于脂肪体、马尔比氏（肾）小管和中肠的不同区域，与解毒过程中的假设作用一致，其他p450表达于性腺、异形体、卵巢细胞、后肠和大脑等器官。结合个体p450的表达模式数据和RNA干扰致死筛选，我们确定了对发育过程至关重要的候选p450，并将它们与具有解毒潜在功能的p450区分开来。
Cytochrome P450s form a large and diverse family of heme-containing proteins capable of carrying out many different enzymatic reactions. In both mammals and plants, some P450s are known to carry out reactions essential for processes such as hormone synthesis, while other P450s are involved in the detoxification of environmental compounds. In general, functions of insect P450s are less well understood. We characterized Drosophila melanogaster P450 expression patterns in embryos and 2 stages of third instar larvae. We identified numerous P450s expressed in the fat body, Malpighian (renal) tubules, and in distinct regions of the midgut, consistent with hypothesized roles in detoxification processes, and other P450s expressed in organs such as the gonads, corpora allata, oenocytes, hindgut, and brain. Combining expression pattern data with an RNA interference lethality screen of individual P450s, we identify candidate P450s essential for developmental processes and distinguish them from P450s with potential functions in detoxification.