Cell type-specific roles for Cdc42, Rac, and RhoL in Drosophila oogenesis.
Cell type-specific roles for Cdc42, Rac, and RhoL in Drosophila oogenesis.
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DOI：
10.1083/jcb.133.3.617
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发表时间：
1996-05
期刊：
影响因子：
--
通讯作者：
Montell DJ
中科院分区：
文献类型：
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作者：
Murphy AM;Montell DJ
已显示GTP酶的Rho亚家族调节细胞形态。我们报告发现的一个新成员的Rho家族，命名为RhoL，这是同样类似的Rac，Rho，和Cdc 42。显性负性RhoL转基因在果蝇卵巢中的表达导致滋养细胞崩溃并融合在一起。Cdc 42的突变形式模仿了这种效应。组成型活性RhoL的表达导致滋养细胞皮层下肌动蛋白的破坏和滋养细胞-卵泡细胞接触的破坏，随后是生殖细胞凋亡。相比之下，Rac活性是一个称为边缘细胞的卵泡细胞亚群迁移所必需的。所有这三种活动都是必要的正常转移的护士细胞质的卵母细胞。这些结果表明，Rho蛋白的活动有细胞类型特异性的影响形态发生。
The Rho subfamily of GTPases has been shown to regulate cellular morphology. We report the discovery of a new member of the Rho family, named RhoL, which is equally similar to Rac, Rho, and Cdc42. Expression of a dominant-negative RhoL transgene in the Drosophila ovary caused nurse cells to collapse and fuse together. Mutant forms of Cdc42 mimicked this effect. Expression of constitutively active RhoL led to nurse cell subcortical actin breakdown and disruption of nurse cell- follicle cell contacts, followed by germ cell apoptosis. In contrast, Rac activity was specifically required for migration of a subset of follicle cells called border cells. All three activities were necessary for normal transfer of nurse cell cytoplasm to the oocyte. These results suggest that Rho protein activities have cell type-specific effects on morphogenesis.