Transpositional reactivation of two LTR retrotransposons in rice-Zizania recombinant inbred lines (RILs).
Transpositional reactivation of two LTR retrotransposons in rice-Zizania recombinant inbred lines (RILs).
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DOI：
10.1111/j.1601-5223.2010.02181.x
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发表时间：
2010-12
期刊：
影响因子：
2.7
通讯作者：
Hongyan Wang;Q. Tian;Yiqiao Ma;Ying Wu;G. Miao;Yan Ma;D. Cao;Xiaoli Wang;Chunjing Lin
中科院分区：
文献类型：
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作者：
Hongyan Wang;Q. Tian;Yiqiao Ma;Ying Wu;G. Miao;Yan Ma;D. Cao;Xiaoli Wang;Chunjing Lin
杂交现象在植物中普遍存在，在基因组进化中起着重要作用。除了通过杂交直接转移和重组产生遗传变异外，该过程本身也可以诱导从头遗传不稳定性。通过种间杂交可以产生这种从头遗传变异的一种机制是在新生杂种中静止的亲本转座因子（TE）的转座再激活。我们以前曾报道过水稻（Oryza sativa L.）Zizania latifolia Griseb诱导了三个TE的猖獗动员，即copia样LTR反转录转座子Tos 17、MITE mPing和属于hAT超家族的II类TE Dart/nDart。在本研究中，我们进一步发现两个额外的LTR反转录转座子，gypsy-like（命名为RIRE 2）和copia-like（命名为Copia 076），也转座激活三个重组自交系（RILs）来自水稻和Z的渐渗杂交。宽叶。这两个逆转录元件在RIL中相对于它们的水稻亲本系（cv. Matsumae）的Southern杂交结果表明，转座子展示（TD）和插入位点的基因座特异性PCR扩增证实了反转录转座。这两个元素被发现转录，但在不同的水平在叶组织的亲本线和RILs，这表明转录控制可能不是一个机制，他们的转座活性的RILs。通过Copia 076对与从头插入相邻的4个基因的表达分析发现，RILs和它们的水稻亲本系之间每个基因的转录丰度存在显著差异，但表达的改变似乎与反转录元件插入无关。
Hybridization is prevalent in plants, which plays important roles in genome evolution. Apart from direct transfer and recombinatory generation of genetic variations by hybridization, de novo genetic instabilities can be induced by the process per se. One mechanism by which such de novo genetic variability can be generated by interspecific hybridization is transpositional reactivation of quiescent parental transposable elements (TEs) in the nascent hybrids. We have reported previously that introgressive hybridization between rice (Oryza sativa L.) and Zizania latifolia Griseb had induced rampant mobilization of three TEs, a copia-like LTR retrotransposon Tos17, a MITE mPing and a class II TE belonging to the hAT superfamily, Dart/nDart. In this study, we further found that two additional LTR retrotransposons, a gypsy-like (named RIRE2) and a copia-like (named Copia076), were also transpositionally reactivated in three recombinant inbred lines (RILs) derived from introgressive hybridization between rice and Z. latifolia. Novel bands of these two retroelements appeared in the RILs relative to their rice parental line (cv. Matsumae) in Southern blot, suggestive of retrotransposition, which was substantiated by transposon display (TD) and locus-specific PCR amplification for insertion sites. Both elements were found to be transcribed but at variable levels in the leaf tissue of the parental line and the RILs, suggesting that transcriptional control was probably not a mechanism for their transpositional activity in the RILs. Expression analysis of four genes adjacent to de novo insertions by Copia076 revealed marked difference in the transcript abundance for each of the genes between the RILs and their rice parental line, but the alterations in expression appeared unrelated with the retroelement insertions.