Chemically defined medium and Caenorhabditis elegans -: art. no. 19
Chemically defined medium and Caenorhabditis elegans -: art. no. 19
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DOI：
10.1186/1472-6750-3-19
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发表时间：
2003-01-01
影响因子：
3.5
通讯作者：
Conley, CA
中科院分区：
文献类型：
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作者：
Szewczyk, NJ;Kozak, E;Conley, CA
背景：C.线虫已经被确立为一个强大的遗传系统。使用化学成分确定的培养基（C. elegans维持培养基（CeMM））现在允许标准化和系统地操作动物接受的营养。液体培养允许自动化的培养和实验，并应使用在大规模的增长和筛选animal.Results：我们发现，CeMM是通用的，培养是简单的。CeMM可以以固体或液体状态使用，它可以储存至少一年不使用，无人看管的活跃生长的培养物可以比标准技术保持更长的时间，并且标准C。elegans方案对于在限定培养基中生长的动物很有效。我们还发现，使用限定培养基有一些注意事项。动物在成分确定的培养基中比在标准培养基中生长更慢，似乎显示出对成分确定的培养基的适应，并且当它们改变成分确定的培养基的组成时显示出改变的生长速率。elegans作为一个潜在的遗传系统，使用确定的培养基与C. elegans应该是一个强大的工具。
Background: C. elegans has been established as a powerful genetic system. Use of a chemically defined medium (C. elegans Maintenance Medium (CeMM)) now allows standardization and systematic manipulation of the nutrients that animals receive. Liquid cultivation allows automated culturing and experimentation and should be of use in large-scale growth and screening of animals.Results: We find that CeMM is versatile and culturing is simple. CeMM can be used in a solid or liquid state, it can be stored unused for at least a year, unattended actively growing cultures may be maintained longer than with standard techniques, and standard C. elegans protocols work well with animals grown in defined medium. We also find that there are caveats to using defined medium. Animals in defined medium grow more slowly than on standard medium, appear to display adaptation to the defined medium, and display altered growth rates as they change the composition of the defined medium.Conclusions: As was suggested with the introduction of C. elegans as a potential genetic system, use of defined medium with C. elegans should prove a powerful tool.