Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening
Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening
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DOI：
10.1136/gut.2008.167817
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发表时间：
2009-10-01
期刊：
影响因子：
24.5
通讯作者：
Sung, J. J. Y.
中科院分区：
文献类型：
--
作者：
Ng, E. K. O.;Chong, W. W. S.;Sung, J. J. Y.
目的：microRNAs（miRNAs）在肿瘤诊断中具有重要的应用价值。方法：本研究分为三个阶段：（1）应用实时荧光定量PCR技术，对5例大肠癌患者的血浆、癌旁组织和癌旁正常结肠组织中的miRNA进行分析，并以5例健康人的血浆作为对照，沿着进行检测;（2）通过实时定量RT-PCR对一小组血浆进行标记物选择和验证;以及（3）对来自90名CRC患者、20名胃癌患者、20名炎性肠病（IBD）患者和50名健康对照的一组血浆进行独立验证。在分析的95种miRNA中，5种在血浆和组织样品中均上调。所有五种miRNA均在25名CRC患者和20名健康对照的血浆中进行了验证。miR-17- 3 p和miR-92在CRC患者中均显著升高（p
Objective: MicroRNAs (miRNAs) have been shown to offer great potential in the diagnosis of cancer. We investigated whether plasma miRNAs could discriminate between patients with and without colorectal cancer (CRC).Methods: This study was divided into three phases: (1) marker discovery using real-time PCR-based miRNA profiling on plasma, corresponding cancerous and adjacent non-cancerous colonic tissues of five patients with CRC, along with plasma from five healthy individuals as controls; (2) marker selection and validation by real-time quantitative RT-PCR on a small set of plasma; and (3) independent validation on a large set of plasma from 90 patients with CRC, 20 patients with gastric cancer, 20 patients with inflammatory bowel disease (IBD) and 50 healthy controls.Results: Of the panel of 95 miRNAs analysed, five were upregulated both in plasma and tissue samples. All the five miRNAs were validated on the plasma of 25 patients with CRC and 20 healthy controls. Both miR-17-3p and miR-92 were significantly elevated in the patients with CRC (p