INTERLEUKIN-6 IS EXPRESSED IN HIGH-LEVELS IN PSORIATIC SKIN AND STIMULATES PROLIFERATION OF CULTURED HUMAN KERATINOCYTES
INTERLEUKIN-6 IS EXPRESSED IN HIGH-LEVELS IN PSORIATIC SKIN AND STIMULATES PROLIFERATION OF CULTURED HUMAN KERATINOCYTES
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DOI：
10.1073/pnas.86.16.6367
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
GOTTLIEB, AB
中科院分区：
文献类型：
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作者：
GROSSMAN, RM;KRUEGER, J;GOTTLIEB, AB
银屑病是一种常见的丘疹性鳞状皮肤病。组织病理学特征为表皮增生和炎症。最近的研究表明，角质形成细胞增殖和炎症在银屑病中是相同的潜在病理过程的表现。白细胞介素6（IL-6）是一种细胞因子，是宿主对组织损伤和感染的反应的主要介质，由培养物中的角质形成细胞和角质形成细胞产生。用两种不同的多克隆抗重组IL-6抗血清免疫过氧化物酶染色和IL-6 cRNA探针原位核酸杂交研究银屑病斑块中IL-6的表达。35例银屑病患者的活动性银屑病斑块中的表皮和真皮细胞与非皮损皮肤和抗代谢药和抗炎药治疗后的斑块相比，IL-6染色严重。用纯化的成纤维细胞来源的IL-6或重组IL-6，但不是牛血清白蛋白的抗重组IL-6抗血清的吸收，去除了免疫染色。通过使用两种不同的生物测定法，在活动性银屑病患者的血浆中检测到IL-6水平升高（平均3 ng/ml）。IL-6的产生增殖角质形成细胞的IL-6特异性免疫染色在培养的正常和银屑病角质形成细胞和银屑病表皮中的IL-6特异性mRNA的原位杂交检测。IL-6刺激培养的正常人角质形成细胞的增殖，通过两种不同的测定法进行评估。因此，IL-6可直接促进银屑病上皮中所见的表皮增生，并影响真皮炎性细胞的功能。
Psoriasis is a common papulosquamous skin disease. The histopathology is characterized by epidermal hyperplasia and inflammation. Recent studies suggest that keratinocyte proliferation and inflammation in psoriasis are manifestations of the same underlying pathological process. Interleukin 6 (IL-6), a cytokine that is a major mediator of the host response to tissue injury and infection, is produced by both keratinocytes and keukocytes in culture. IL-6 expression was studied in psoriatic plaques by immunoperoxidase staining with two different polyclonal anti-recombinant IL-6 antisera and by in situ nucleic acid hybridization with IL-6 cRNA probes. Epidermal and dermal cells in active psoriatic plaques from 35 psoriasis patients stained heavily for IL-6 as compared with nonlesional skin and with plaques after treatment with antimetabolic and antiinflammatory agents. Absorption of the anti-recombinant IL-6 antisera with purified fibroblast-derived IL-6 or with recombinant IL-6, but not bovine serum albumin, removed the immunostaining. Increased levels of IL-6 were detected in the plasma of patients with active psoriasis (mean 3 ng/ml) by using two different bioassays. IL-6 production by proliferating keratinocytes was suggested by IL-6-specific immunostaining in cultured normal and psoriatic keratinocytes and by the detection of mRNA specific for IL-6 in psoriatic epidermis by in situ hybridization. IL-6 stimulated the proliferation of cultured, normal human keratinocytes as assessed by two different assays. Thus, IL-6 could directly contribute to the epidermal hyperplasia seen in psoriatic epithelium as well as affect the function of dermal inflammatory cells.