The influence of controlled surface nanotopography on the early biological events of osseointegration
The influence of controlled surface nanotopography on the early biological events of osseointegration
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DOI：
10.1016/j.actbio.2017.02.026
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发表时间：
2017-04-15
影响因子：
9.7
通讯作者：
Omar, Omar
中科院分区：
文献类型：
--
作者：
Karazisis, Dimitrios;Petronis, Sarunas;Omar, Omar
早期细胞和组织与纳米图案化钛植入物的相互作用在体内描述不足。一个限制是将预定的、良好控制的纳米形貌转移到3D钛植入物，而不影响其他表面参数，包括表面微观形貌和化学。该体内研究旨在研究叠加有受控纳米形貌的螺旋形钛植入物骨界面的早期细胞和分子事件。抛光和机加工的钛种植体首先与75纳米的半球形突起图案化。没有纳米图案的抛光和机加工的植入物被指定为对照。此后，所有纳米图案化和对照植入物均涂覆有30 nm钛层以统一表面化学。将种植体植入大鼠胫骨，并在12 h、1 d和3 d后收获样品。在一组中，种植体被拧松，种植体粘附细胞进行了分析，使用定量聚合酶链反应。在另一组中，将种植体与周围骨一起整块收获用于组织学和免疫组织化学。结果表明，纳米形貌术在1 d时下调单核细胞趋化蛋白-1（MCP-1）的表达，在3 d时触发骨钙素（DC）的表达。这与纳米图案化植入物周围组织中招募的CD 68阳性巨噬细胞数量相对较低平行。此外，在纳米图案化的植入物中，在3d时发现新形成的类骨质和编织骨的比例更高。得出的结论是，nanotopography，本身，减弱炎症过程中，增强骨整合的早期阶段的成骨反应。这种nanotopography-induced效果似乎是独立的基础microscale topography.Statement的显著性这项研究提供了第一条线的证据表明，预先确定的nanopatrium对临床相关的，螺旋形，钛植入物可以识别的细胞在复杂的体内环境。到目前为止，大部分与细胞与纳米图案化表面相互作用有关的知识都是从体外研究中获得的，这些研究主要涉及不同化学成分的二维纳米图案化表面。我们已经成功地在抛光和微粗糙的螺旋形植入物上形成了预定的纳米级形貌，而没有改变微米级形貌或化学性质。这是通过胶体光刻结合纳米图案化和对照植入物顶部的薄钛膜涂层来实现的。在这些种植体的骨界面评价骨整合的早期事件。结果表明，纳米形貌，因此，elevenness下调的影响，早期招聘和活动的炎性细胞，同时提高成骨活性和编织骨形成。(C)2017 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The early cell and tissue interactions with nanopatterned titanium implants are insufficiently described in vivo. A limitation has been to transfer a pre-determined, well-controlled nanotopography to 3D titanium implants, without affecting other surface parameters, including surface microtopography and chemistry. This in vivo study aimed to investigate the early cellular and molecular events at the bone interface with screw-shaped titanium implants superimposed with controlled nanotopography. Polished and machined titanium implants were firstly patterned with 75-nm semispherical protrusions. Polished and machined implants without nano-patterns were designated as controls. Thereafter, all nanopatterned and control implants were sputter-coated with a 30 nm titanium layer to unify the surface chemistry. The implants were inserted in rat tibiae and samples were harvested after 12 h,1 d and 3 d. In one group, the implants were unscrewed and the implant-adherent cells were analyzed using quantitative polymerase chain reaction. In another group, implants with surrounding bone were harvested en bloc for histology and immunohistochemistry. The results showed that nanotopography downregulated the expression of monocyte chemoattractant protein-1 (MCP-1), at 1 d, and triggered the expression of osteocalcin (DC) at 3 d. This was in parallel with a relatively lower number of recruited CD68-positive macrophages in the tissue surrounding the nanopatterned implants. Moreover, a higher proportion of newly formed osteoid and woven bone was found at the nanopatterned implants at 3 d. It is concluded that nanotopography, per se, attenuates the inflammatory process and enhances the osteogenic response during the early phase of osseointegration. This nanotopography-induced effect appeared to be independent of the underlying microscale topography.Statement of SignificanceThis study provides a first line of evidence that pre-determined nanopatterns on clinically relevant, screw-shaped, titanium implants can be recognized by cells in the complex in vivo environment. Until now, most of the knowledge relating to cell interactions with nanopatterned surfaces has been acquired from in vitro studies involving mostly two-dimensional nanopatterned surfaces of varying chemical composition. We have managed to superimpose pre-determined nanoscale topography on polished and micro-rough, screw-shaped, implants, without changes in the microscale topography or chemistry. This was achieved by colloidal lithography in combination with a thin titanium film coating on top of both nanopatterned and control implants. The early events of osseointegration were evaluated at the bone interface to these implants. The results revealed that nanotopography, as such, elicits downregulatory effects on the early recruitment and activity of inflammatory cells while enhancing osteogenic activity and woven bone formation. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.