Development of a multilocus sequence typing scheme for Ureaplasma
Development of a multilocus sequence typing scheme for Ureaplasma
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解脲支原体多位点序列分型方案的开发
DOI：
10.1007/s10096-013-1981-y
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发表时间：
2014-04-01
影响因子：
4.5
通讯作者：
Xie, X.
中科院分区：
文献类型：
--
作者：
Zhang, J.;Kong, Y.;Xie, X.
脲原体是泌尿生殖道感染的主要病原体，但常与非淋菌性尿道炎、不孕症等侵袭性疾病及不良妊娠结局相关。为了更好地了解脲原体的分子流行病学和群体结构，建立了基于ftsH、rpL22、valS、thrS四个管家基因的多位点序列分型（MLST）方法，并对283株脲原体进行了验证。共发现99个序列型（ST），其中14个血清型菌株归属于12个ST，临床分离株中出现87个新的和特殊的ST。ST 1和ST 2 2是优势ST，分别含有68株和70株。eBURST分析显示了两个克隆谱系（CC1和CC2），通过标准化关联指数（I（A）（S））揭示了连锁不平衡。14个脲原体血清型的邻接树结果显示两个遗传距离显著的聚类，与脲原体的种分类[微小脲原体（UPA）和解脲脲原体（UUR）]高度一致。对269株临床分离菌进行生物型分析，CC1全部为UPA，CC2全部为UUR。此外，CC2更常见于有阴道炎、输卵管阻塞和宫颈炎症状的患者。总之，这种MLST方案是足够的分子流行病学和人口结构的调查具有高度的区分能力。
Ureaplasma is a commensal of the human urogenital tract but is always associated with invasive diseases such as non-gonococcal urethritis and infertility adverse pregnancy outcomes. To better understand the molecular epidemiology and population structure of Ureaplasma, a multilocus sequence typing (MLST) scheme based on four housekeeping genes (ftsH, rpL22, valS, thrS) was developed and validated using 283 isolates, including 14 serovars of reference strains and 269 strains obtained from clinical patients. A total of 99 sequence types (STs) were revealed: the 14 type strains of the Ureaplasma serovars were assigned to 12 STs, and 87 novel and special STs appeared among the clinical isolates. ST1 and ST22 were the predominant STs, which contained 68 and 70 isolates, respectively. Two clonal lineages (CC1 and CC2) were shown by eBURST analysis, and linkage disequilibrium was revealed through a standardized index of association (I (A) (S)). The neighbor-joining tree results of 14 Ureaplasma serovars showed two genetically significantly distant clusters, which was highly congruent with the species taxonomy of ureaplasmas [Ureaplasma parvum (UPA) and Ureaplasma urealyticum (UUR)]. Analysis of the biotypes of 269 clinical isolates revealed that all the isolates of CC1 were UPA and those of CC2 were UUR. Additionally, CC2 was found more often in symptomatic patients with vaginitis, tubal obstruction, and cervicitis. In conclusion, this MLST scheme is adequate for investigations of molecular epidemiology and population structure with highly discriminating capacity.