EGF INHIBITS THE DIFFERENTIATION OF ADIPOCYTE PRECURSORS IN PRIMARY CULTURES
EGF INHIBITS THE DIFFERENTIATION OF ADIPOCYTE PRECURSORS IN PRIMARY CULTURES
复制标题
DOI：
10.1016/0006-291x(87)90710-8
复制
发表时间：
1987-07-15
影响因子：
3.1
通讯作者：
SERRERO, G
中科院分区：
文献类型：
--
作者：
SERRERO, G
从2日龄NBR大鼠的腹股沟脂肪垫中分离出脂肪细胞前体，在特定的培养液中增殖和分化。EGF以2 ng/ml的ED50刺激脂肪前体细胞的增殖，以0.45 ng/ml的ED50抑制脂肪细胞的分化，对分化的脂肪细胞无影响。EGF结合研究表明，脂肪细胞前体有两类受体(Kd4倍)。10-11M，4800受体/细胞，Kd1.4倍。10-9M，22,000个受体/细胞)。TFG-.阿尔法。对脂肪细胞前体细胞的作用与EGF相同。相反，碱性和酸性成纤维细胞生长因子促进脂肪细胞前体的增殖，但不抑制其分化。
Adipocyte precursors, isolated from inguinal fat pads of 2-day old NBR rats, proliferate and differentiate in defined medium. EGF stimulates the proliferation of adipocyte precursors with an ED50 of 2 ng/ml and inhibits their differentiation with an ED50 of 0.45 ng/ml. EGF has no effect on differentiated adipocytes. EGF binding studies indicate that adipocyte precursors have two classes of receptors (Kd 4 .times. 10-11 M, 4800 receptors/cell and Kd 1.4 .times. 10-9 M, 22,000 receptors/cell). TFG-.alpha. had the same effect as EGF on adipocyte precursors. In contrast, basic and acidic FGF stimulate adipocyte precursor proliferation without inhibiting their differentiation.