C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization.
C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization.
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DOI：
10.1002/jcp.25062
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发表时间：
2016-01
影响因子：
5.6
通讯作者：
Stein GS
中科院分区：
文献类型：
--
作者：
Barutcu AR;Fritz AJ;Zaidi SK;van Wijnen AJ;Lian JB;Stein JL;Nickerson JA;Imbalzano AN;Stein GS
染色质的三维组织在转录、复制、DNA修复和病理事件如易位中具有重要作用。有两种基本的方法来研究高级染色质组织：显微镜和分子方法。在这篇综述中，我们简要介绍了分子方法，重点是染色体构象捕获或“3C”技术及其衍生物，它可以用来探测染色质折叠的分辨率超出了显微镜技术提供的。我们进一步讨论了基于3C的方法产生的不同类型的数据，以及它们如何用于回答不同的生物学问题。
Three-dimensional organization of the chromatin has important roles in transcription, replication, DNA repair, and pathologic events such as translocations. There are two fundamental ways to study higher-order chromatin organization: microscopic and molecular approaches. In this review, we briefly introduce the molecular approaches, focusing on chromosome conformation capture or “3C” technology and its derivatives, which can be used to probe chromatin folding at resolutions beyond that provided by microscopy techniques. We further discuss the different types of data generated by the 3C-based methods and how they can be used to answer distinct biological questions.