Meiotic DNA breaks associated with recombination in S. pombe
Meiotic DNA breaks associated with recombination in S. pombe
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DOI：
10.1016/s1097-2765(00)80328-7
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发表时间：
2000-05-01
期刊：
影响因子：
16
通讯作者：
Smith, GR
中科院分区：
文献类型：
--
作者：
Cervantes, MD;Farah, JA;Smith, GR
在分裂酵母裂糖酵母pombe中，我们已经检测到在减数分裂DNA复制后不久出现的显著DNA断裂。这些断裂，就像减数分裂重组一样，需要测试六个rec基因的产物。在相距约100-300 kb的较宽的位点上检测到明显的断裂，相当于每个基因组约50-150个位点或大约减数分裂重组事件的数量。这些断裂的某些特征与远亲酵母酿酒酵母相似，酿酒酵母是唯一一种有减数分裂DNA断裂报道的生物。然而，其他特征似乎有所不同。这些结果表明，虽然DNA断裂可能是减数分裂重组的普遍特征，但pombe的断裂可能起着不同于S. cerevisiae的作用。
In the fission yeast Schizosaccharomyces pombe, we have detected prominent DNA breaks that appeared shortly after premeiotic DNA replication. These breaks, like meiotic recombination, required the products of the six rec genes tested. Prominent breaks were detected at widely separated sites, about 100-300 kb apart, equivalent to about 50-150 sites per genome or approximately the number of meiotic recombination events. Certain features of these breaks are similar to those in the distantly related yeast Saccharomyces cerevisiae, the only other organism in which meiotic DNA breaks have been reported. Other features, however, appear to be different. These results suggest that, although DNA breaks may be a general feature of meiotic recombination, the breaks in S. pombe may play a role different from those in S. cerevisiae.