Phase-contrast computed tomography for quantification of structural changes in lungs of asthma mouse models of different severity.
Phase-contrast computed tomography for quantification of structural changes in lungs of asthma mouse models of different severity.
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DOI：
10.1107/s1600577515006177
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发表时间：
2015-07
影响因子：
2.5
通讯作者：
Alves F
中科院分区：
文献类型：
--
作者：
Dullin C;Larsson E;Tromba G;Markus AM;Alves F
同步加速器内联相衬计算机断层扫描结合单距离相位检索，使定量的形态学改变与轻度和重度实验性过敏性气道疾病的小鼠与健康对照组相比，肺。小鼠疾病模型中的肺成像对于评估气道疾病的严重程度至关重要，但由于器官的小尺寸和高孔隙率，仍然具有挑战性。同步加速器内联自由传播相衬计算机断层扫描（CT）具有固有的高软组织对比度，提供了必要的灵敏度和空间分辨率，以分析小鼠肺结构的细节。在这里，这种技术已被应用于与单距离相位检索相结合，以量化不同严重程度的实验性哮喘小鼠模型的肺结构的改变。为了尽可能接近地模拟体内情况，在恒定的生理压力下用空气对肺进行充气。将整个小鼠包埋在琼脂糖凝胶中，并在SYRMEP光束线（同步加速器光源，'Elettra'，的里雅斯特，意大利）上使用在线自由传播相衬CT进行成像。所获得的相衬CT数据集的定量显示，小鼠肺软组织含量的增加与实验性过敏性气道疾病的严重程度相关。通过这种方式，可以成功区分健康对照组和患有轻度或重度过敏性气道疾病的小鼠。据信，这种方法可能有潜力评估新的治疗策略，目标气道重塑过程中的哮喘的疗效。
Synchrotron inline phase-contrast computed tomography in combination with single-distance phase retrieval enables quantification of morphological alterations in lungs of mice with mild and severe experimental allergic airways disease in comparison with healthy controls. Lung imaging in mouse disease models is crucial for the assessment of the severity of airway disease but remains challenging due to the small size and the high porosity of the organ. Synchrotron inline free-propagation phase-contrast computed tomography (CT) with its intrinsic high soft-tissue contrast provides the necessary sensitivity and spatial resolution to analyse the mouse lung structure in great detail. Here, this technique has been applied in combination with single-distance phase retrieval to quantify alterations of the lung structure in experimental asthma mouse models of different severity. In order to mimic an in vivo situation as close as possible, the lungs were inflated with air at a constant physiological pressure. Entire mice were embedded in agarose gel and imaged using inline free-propagation phase-contrast CT at the SYRMEP beamline (Synchrotron Light Source, ‘Elettra’, Trieste, Italy). The quantification of the obtained phase-contrast CT data sets revealed an increasing lung soft-tissue content in mice correlating with the degree of the severity of experimental allergic airways disease. In this way, it was possible to successfully discriminate between healthy controls and mice with either mild or severe allergic airway disease. It is believed that this approach may have the potential to evaluate the efficacy of novel therapeutic strategies that target airway remodelling processes in asthma.