THE EFFECT OF QUIN2 ON CHEMOTAXIS BY POLYMORPHONUCLEAR LEUKOCYTES
THE EFFECT OF QUIN2 ON CHEMOTAXIS BY POLYMORPHONUCLEAR LEUKOCYTES
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DOI：
10.1016/0167-4889(85)90007-2
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
DEIERKAUF, M
中科院分区：
文献类型：
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作者：
ELFERINK, JGR;DEIERKAUF, M
暴露于微摩尔浓度的quin 2-AM的兔多形核白细胞导致quin 2的高细胞内浓度，这导致对趋化性的抑制。多形核白细胞的装载效率，即被细胞摄取并在细胞内转化为quin 2的quin 2-AM的百分比，非常高，在30分钟后达到最大值，不依赖于细胞外Ca 2+的存在，并且相当不依赖于细胞浓度。因此，趋化性的抑制强烈依赖于实验条件：在低细胞密度（3. 106/min）暴露于20 μ M quin 2-AM导致趋化性的完全抑制，而当使用8倍更高的细胞浓度时，相同浓度的quin 2-AM几乎没有作用。quin 2的抑制依赖于细胞外Ca 2 +;在细胞外Ca 2+不存在的情况下，抑制比在其存在下更明显。这表明quin 2通过干扰细胞内Ca 2+来抑制趋化性。
Exposure of rabbit polymorphonuclear leukocytes to micromolar concentrations of quin 2-AM results in high intracellular concentrations of quin2, which lead to inhibition of chemotaxis. The loading efficiency of polymorphonuclear leukocytes, being the percentage of quin2-AM which is taken up by the cells and transformed intracellularly into quin2, is very high, reaches a maximum after 30 min, is independent of the presence of extracellular Ca2+ and is fairly independent of cell concentration. As a consequence, inhibition of chemotaxis is strongly dependent on experimental conditions: with a low cell density (3 .cntdot. 106/min) exposure to 20 .mu.M quin2-AM results in complete inhibition of chemotaxis, whereas the same concentration of quin2-AM is nearly without effect when an 8-fold higher cell concentration is used. Inhibition by quin2 is dependent on extracellular Ca2+; inhibition is more pronounced in the absence of extracellular Ca2+ than in its presence. It is suggested that quin2 inhibits chemotaxis by interference with intracellular Ca2+.