Innate immunity is sufficient for the clearance of Chlamydia trachomatis from the female mouse genital tract.
Innate immunity is sufficient for the clearance of Chlamydia trachomatis from the female mouse genital tract.
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DOI：
10.1111/2049-632x.12164
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发表时间：
2014-10
影响因子：
3.3
通讯作者：
Caldwell HD
中科院分区：
文献类型：
--
作者：
Sturdevant GL;Caldwell HD
分别用小鼠和人两种鼠株墨氏衣原体和沙眼衣原体在小鼠雌性生殖道感染模型中进行了免疫研究。尽管有证据表明，这些基因组相似的菌株的独特基因可能在它们各自的小鼠和人类宿主的先天免疫逃避中发挥作用，但没有动物模型的发现直接支持这一结论。在这里，我们用这些菌株感染C57BL/6和适应性免疫缺陷的Rag1−/−雌性小鼠，并评估它们自发解决生殖器感染的能力。可以预见的是，C57BL/6小鼠自发清除了两种衣原体菌株引起的感染。相比之下，Rag1−/−小鼠缺乏成熟T细胞和B细胞免疫，但保持功能性先天免疫效应，无法解决muridarum感染，但自发清除沙眼衣原体感染。小鼠和人类菌株在适应性免疫和先天免疫介导的清除方面的这种截然不同的差异，对小鼠模型中自然免疫和疫苗开发的研究具有重要的警示意义。
Chlamydia muridarum and C. trachomatis, mouse and human strains respectively, have been used to study immunity in a murine model of female genital tract infection. Despite evidence that unique genes of these otherwise genomically similar strains could play a role in innate immune evasion in their respective mouse and human hosts there have been no animal model findings to directly support this conclusion. Here, we infected C57BL/6 and adaptive immune deficient Rag1−/− female mice with these strains and evaluated their ability to spontaneously resolve genital infection. Predictably, C57BL/6 mice spontaneously cleared infection caused by both chlamydial strains. In contrast, Rag1−/− mice which lack mature T and B cell immunity but maintain functional innate immune effectors, were incapable of resolving C. muridarum infection but spontaneously cleared C. trachomatis infection. This distinct dichotomy in adaptive and innate immune-mediated clearance between mouse and human strains has important cautionary implications for the study of natural immunity and vaccine development in the mouse model.