Hypoxia-Selective DNA Interstrand Cross-Link Formation by Two Modified Nucleosides
Hypoxia-Selective DNA Interstrand Cross-Link Formation by Two Modified Nucleosides
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DOI：
10.1002/chem.201201960
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发表时间：
2012-10-01
影响因子：
4.3
通讯作者：
Peng, Xiaohua
中科院分区：
文献类型：
--
作者：
Kuang, Yunyan;Sun, Huabing;Peng, Xiaohua
合成了两个硝基咪唑修饰的胸苷1a和1b，并将其掺入DNA寡聚体中。1a和1b的350 nm光解产生5-（2′-脱氧尿苷基）甲基自由基，该自由基诱导DNA链间交联（ICL）。在低氧条件下比有氧条件下观察到更高的ICL产率。单体或DNA寡聚体的光产物被分离，并通过NMR和/或质谱进行表征。
Two nitroimidazole modified thymidines 1a and 1b have been synthesized and incorporated into DNA oligomers. The 350 nm photolysis of 1a and 1b generated a 5-(2′-deoxyuridinyl)methyl radical that induced DNA interstrand cross-links (ICL). Higher ICL yield was observed under hypoxic conditions than aerobic conditions. The photoproducts of monomers or DNA oligomers were isolated and characterized by NMR and/or mass spectroscopy.