Sensitive analysis of ethanolamine- and serine-containing phosphoglycerides by high-performance liquid chromatography.
Sensitive analysis of ethanolamine- and serine-containing phosphoglycerides by high-performance liquid chromatography.
复制标题
通过高效液相色谱法对含乙醇胺和丝氨酸的磷酸甘油酯进行灵敏分析。
DOI：
10.1042/bj1450517
复制
发表时间：
1975
期刊：
影响因子：
--
通讯作者：
R. McCluer
中科院分区：
文献类型：
--
作者：
F. B. Jungalwala;R. J. Turel;J. Evans;R. McCluer
本文描述了一种高灵敏度的方法，用于分离和定量测定含有初级氨基的磷脂，如磷脂酰乙醇胺、磷脂酰丝氨酸和溶血磷脂酰乙醇胺。该方法涉及将含有氨基的磷脂简单定量地生成其紫外吸收联苯羰基衍生物。它们在268nm处的摩尔消光系数约为23000。然后将磷脂衍生物分离并通过高效液相色谱法进行非破坏性测定。含有乙烯醚键的氨基磷脂（等离子原）可与二酰基和烷基酰基氨基磷脂分开测定。磷脂衍生物的高效液相色谱分析检测下限约为10-13pmol或0.3-0.4ng磷脂P.含有氨基的磷脂衍生物形成和高效液相色谱分析的定量范围为10-500nmol。结果表明，该方法适用于组织样品中含氨基磷脂的分析。
A highly sensitive method for the separation and quantitative measurement of phospholipids containing primary amino groups, such as phosphatidylethanolamine, phosphatidylserine and lysophosphatidylethanolamine, is described. The method involves a simple and quantitative derivative formation of the phospholipids containing amino groups to their u.v.-absorbing biphenylcarbonyl derivatives. These have molar extinction coefficients of about 23,000 at 268nm. The phospholipid derivatives are then separated and non-destructively determined by high-performance liquid chromatography. The amino phospholipids containing vinyl ether bonds (plasmalogens) can be determined separately from the diacyl- and alkylacyl-amino phospholipids. The lower limit of detection by high-performance liquid-chromatographic analysis of the phospholipid derivatives is about 10-13pmol or 0.3-0.4ng of phospholipid P. The quantitative range of derivative formation and analysis by high-performance liquid chromatography of the phospholipids containing amino groups was shown to be 10-500nmol. The method was shown to be applicable to the analysis of phospholipids containing amino groups in tissue samples.