The BMP1 gene is essential for pathogenicity in the gray mold fungus Botrytis cinerea.
The BMP1 gene is essential for pathogenicity in the gray mold fungus Botrytis cinerea.
复制标题
DOI：
10.1094/mpmi.2000.13.7.724
复制
发表时间：
2000-07
期刊：
影响因子：
--
通讯作者：
Li Zheng;Mathew Campbell;J. Murphy;Stephen Lam;Jin-Rong Xu
中科院分区：
文献类型：
--
作者：
Li Zheng;Mathew Campbell;J. Murphy;Stephen Lam;Jin-Rong Xu
在稻瘟病菌中，一个高度保守的丝裂原活化蛋白（MAP）激酶基因PMK1是真菌致病所必需的。在这项研究中，我们测试了相同的MAP激酶是否是灰霉病菌Botrytis cinerea感染植物所必需的，Botrytis cinerea是一种坏死型病原体，其感染机制不同于M。稻瘟病菌我们使用了聚合酶链反应为基础的方法来分离MAP激酶同源物从B。灰色区域。葡萄孢霉MAP激酶基因与M.稻瘟病菌PMK1. BMP 1是一个单拷贝基因。BMP 1基因置换突变体产生正常的分生孢子和菌丝体，但在营养丰富的培养基上生长速率降低。bmp 1突变体对香石竹花和番茄叶片无致病性。将野生型BMP 1等位基因重新引入bmp 1突变体中，恢复了正常的生长速率和致病性。进一步的研究表明，bmp1突变体的分生孢子能在植物表面萌发，但不能穿透和浸渍植物组织。BMP 1突变体在通过伤口感染方面似乎也有缺陷。这些结果表明，BMP 1是B中植物侵染所必需的。灰霉病，这种MAP激酶途径可能在病原真菌中广泛保守，用于调节感染过程。
In Magnaporthe grisea, a well-conserved mitogen-activated protein (MAP) kinase gene, PMK1, is essential for fungal pathogenesis. In this study, we tested whether the same MAP kinase is essential for plant infection in the gray mold fungus Botrytis cinerea, a necrotrophic pathogen that employs infection mechanisms different from those of M. grisea. We used a polymerase chain reaction-based approach to isolate MAP kinase homologues from B. cinerea. The Botrytis MAP kinase required for pathogenesis (BMP) MAP kinase gene is highly homologous to the M. grisea PMK1. BMP1 is a single-copy gene. bmp1 gene replacement mutants produced normal conidia and mycelia but were reduced in growth rate on nutrient-rich medium. bmp1 mutants were nonpathogenic on carnation flowers and tomato leaves. Re-introduction of the wild-type BMP1 allele into the bmp1 mutant restored both normal growth rate and pathogenicity. Further studies indicated that conidia from bmp1 mutants germinated on plant surfaces but failed to penetrate and macerate plant tissues. bmp1 mutants also appeared to be defective in infecting through wounds. These results indicated that BMP1 is essential for plant infection in B. cinerea, and this MAP kinase pathway may be widely conserved in pathogenic fungi for regulating infection processes.