Chromosome-designated mutation selection in Tetrahymena thermophila.
Chromosome-designated mutation selection in Tetrahymena thermophila.
复制标题
嗜热四膜虫的染色体指定突变选择。
DOI：
10.1093/genetics/106.3.387
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Bruns,PJ
中科院分区：
文献类型：
--
作者：
Altschuler,MI;Bruns,PJ
提出了两个方案，允许选择映射到四膜虫嗜热微核染色体5的突变。一种方案涉及将诱变的二倍体细胞直接与5号染色体的缺体菌株杂交，并筛选单体后代的突变表型。第二种方案首先将诱变的二倍体细胞通过第一轮基因组排除来创建有用的和可重复使用的突变异核体，然后通过与缺体5菌株杂交来检测这些异核体在5号染色体上是否存在突变。在这两种方法获得的14个可能的5号染色体突变中，遗传分析显示有7个是位于5号染色体上的隐性突变；1个位于基因组的其他地方；6个在一些诊断杂交中是不育或未能产生后代的，因此，它们的遗传性质无法确定。
Two protocols are presented that allow the selection of mutations mapping to micronuclear chromosome5inTetrahymena thermophilia. One protocol involves crossing mutagenized diploid cells directly to a strain nullisomic for chromosome5and screening the monosomic progeny for a mutant phenotype. The second protocol first takes the mutagenized diploid cells through round I of genomic exclusion to create useful and reusable mutant heterokaryons, which are then assayed for the presence of mutations on chromosome5by crossing to the nullisomic5strain. Of 14 putative chromosome5mutations obtained by these two methods, seven are shown by genetic analysis to be recessive mutations on chromosome5; one mapped elsewhere in the genome; six were infertile or failed to yield progeny in some of the diagnostic crosses and, thus, their genetic nature could not be determined with certainty.