Hsp90 and the quantitative variation of wing shape in Drosophila melanogaster
Hsp90 and the quantitative variation of wing shape in Drosophila melanogaster
复制标题
DOI：
10.1554/06-045.1
复制
发表时间：
2006-12-01
期刊：
影响因子：
3.3
通讯作者：
Hoffmann, Ary A.
中科院分区：
文献类型：
--
作者：
Debat, Vincent;Milton, Claire C.;Hoffmann, Ary A.
分子伴侣蛋白 Hsp90 作为发育缓冲的候选基因已被广泛讨论。我们利用几何形态计量学的方法分析了其对果蝇个体变异和翅形波动不对称性的影响。使用三种不同的实验方法来降低 Hsp90 活性。在第一个实验中，发育中的幼虫在含有 Hsp90 特异性抑制剂格尔德霉素的食物中饲养，但个体差异和波动不对称性都没有改变。另外两项实验产生了基因相同的果蝇品系，它们携带 Hsp83（编码 Hsp90 蛋白的基因）突变，在九种不同的遗传背景下处于杂合状态。其中第一个，引入携带两个 Hsp83 突变之一的整个染色体，在九个遗传背景中的任何一个中，与野生型对照相比，都没有增加形状变异或不对称性。相比之下，在第三个实验中，其中一个 Hsp83 等位基因渗入到作为对照的野生型背景中，诱导了九个遗传背景中每一个的个体变异和波动不对称性的增加。没有检测到 Hsp90 对品系差异的影响，因此没有为翅膀形状的隐秘遗传变异提供证据。总体而言，这些结果表明 Hsp90 有助于但不控制翅形状表型变异的缓冲。
The molecular chaperone protein Hsp90 has been widely discussed as a candidate gene for developmental buffering. We used the methods of geometric morphometrics to analyze its effects on the variation among individuals and fluctuating asymmetry of wing shape in Drosophila melanogaster. Three different experimental approaches were used to reduce Hsp90 activity. In the first experiment, developing larvae were reared in food containing a specific inhibitor of Hsp90, geldanamycin, but neither individual variation nor fluctuating asymmetry was altered. Two further experiments generated lines of genetically identical flies carrying mutations of Hsp83, the gene encoding the Hsp90 protein, in heterozygous condition in nine different genetic backgrounds. The first of these, introducing entire chromosomes carrying either of two Hsp83 mutations, did not increase shape variation or asymmetry over a wild-type control in any of the nine genetic backgrounds. In contrast, the third experiment, in which one of these Hsp83 alleles was introgressed into the wild-type background that served as the control, induced an increase in both individual variation and fluctuating asymmetry within each of the nine genetic backgrounds. No effect of Hsp90 on the difference among lines was detected, providing no evidence for cryptic genetic variation of wing shape. Overall, these results suggest that Hsp90 contributes to, but is not controlling, the buffering of phenotypic variation in wing shape.