Counting alleles to predict recurrence of early-stage colorectal cancers
Counting alleles to predict recurrence of early-stage colorectal cancers
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DOI：
10.1016/s0140-6736(02)07448-2
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发表时间：
2002-01-19
期刊：
影响因子：
168.9
通讯作者：
Vogelstein, B
中科院分区：
文献类型：
--
作者：
Zhou, W;Goodman, SN;Vogelstein, B
背景 染色体失衡发生在许多癌症中，代表了肿瘤的重要生物学特性。然而，测量这种不平衡是很困难的。我们使用了一种新的定量方法来研究染色体失衡对早期结直肠癌的预后价值。方法我们研究了 180 名手术时没有淋巴结或远处转移证据的患者。通过数字 SNP（单核苷酸多态性）测试来自石蜡包埋肿瘤的 DNA 的染色体 8p 和 18q 不平衡情况，该技术直接对样本中的每个等位基因进行计数。存活患者的中位随访时间为 68 个月，疾病复发被用作临床终点。 结果 肿瘤分为三组：“L”肿瘤（n=93）具有 8p 和 18q 染色体等位基因不平衡，“L/R”肿瘤（n=60）具有 8p 或 18q 染色体等位基因不平衡，但不是两者均存在，“R”肿瘤（n=27）保留了染色体 8p 和 18q 等位基因平衡。染色体。 R肿瘤患者的5年无病生存率为100%（95% CI 80-100），L/R肿瘤患者的5年无病生存率为74%（61-87），L肿瘤患者的5年无病生存率为58%（47-69）。这些差异是显着的（p
Background Chromosome imbalances occur in many cancers and represent important biological properties of tumours. However, measurements of such imbalances are difficult. We used a new, quantitative approach to investigate the prognostic value of chromosome imbalances in early-stage colorectal cancers.Methods We studied 180 patients with no evidence of lymph-node or distant metastases at the time of surgery. DNA from paraffin-embedded tumours was tested for imbalances of chromosome 8p and 18q by digital SNP (single-nucleotide polymorphism)-a technique in which each allele in a sample is directly counted. Surviving patients had median follow-up of 68 months, and disease recurrence was used as the clinical endpoint.Findings Tumours were divided into three groups: "L" tumours (n=93) had allelic imbalances of chromosomes 8p and 18q, "L/R" tumours (n=60) had allelic imbalances of either chromosome 8p or 18q but not both, and "R" tumours (n=27) retained allelic balance for both chromosomes. 5-year disease-free survival was 100% (95% CI 80-100) for patients with R tumours, 74% (61-87) for patients with L/R tumours, and 58% (47-69) for those with L tumours. These differences were significant (p