Making Mass Spectrometry See the Light: The Promises and Challenges of Cryogenic Infrared Ion Spectroscopy as a Bioanalytical Technique.
Making Mass Spectrometry See the Light: The Promises and Challenges of Cryogenic Infrared Ion Spectroscopy as a Bioanalytical Technique.
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DOI：
10.1007/s13361-016-1366-4
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发表时间：
2016-05
影响因子：
3.2
通讯作者：
Polfer NC
中科院分区：
文献类型：
--
作者：
Cismesia AP;Bailey LS;Bell MR;Tesler LF;Polfer NC
低温冷却分析物的振动光谱中所包含的详细化学信息，原则上会使红外离子光谱成为质谱中分子鉴定的金标准技术。尽管具有这种巨大潜力，但在该技术具有分析实用性之前，在仪器和方法两方面都有相当大的挑战需要克服。在此，我们在代谢物鉴定的背景下讨论红外离子光谱用于小分子分析的前景。解决灵敏度受限、总体占空比不佳以及实验速度问题的实验策略与质量选择低温阱的开发密切相关。因此，作者在此提出了最有可能成功的途径，以及替代方法和一些关于数据解读的思考。
The detailed chemical information contained in the vibrational spectrum of a cryogenically cooled analyte would, in principle, make infrared (IR) ion spectroscopy a gold standard technique for molecular identification in mass spectrometry. Despite this immense potential, there are considerable challenges in both instrumentation and methodology to overcome before the technique is analytically useful. Here, we discuss the promise of IR ion spectroscopy for small molecule analysis in the context of metabolite identification. Experimental strategies to address sensitivity constraints, poor overall duty cycle, and speed of the experiment are intimately tied to the development of a mass-selective cryogenic trap. Therefore, the most likely avenues for success, in the authors? opinion, are presented here, alongside alternative approaches and some thoughts on data interpretation.