Utilizing the micron sized non-thermal atmospheric pressure plasma inside the animal body for the tumor treatment application.
Utilizing the micron sized non-thermal atmospheric pressure plasma inside the animal body for the tumor treatment application.
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DOI：
10.1038/srep29048
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发表时间：
2016-07-07
影响因子：
4.6
通讯作者：
Nikkhah M
中科院分区：
文献类型：
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作者：
Mirpour S;Piroozmand S;Soleimani N;Jalali Faharani N;Ghomi H;Fotovat Eskandari H;Sharifi AM;Mirpour S;Eftekhari M;Nikkhah M
本研究旨在探讨微米级低温常压等离子体在动物体内对乳腺癌肿瘤的治疗作用。μ-等离子体射流由微米级的空心管组成，其中纯氦气在高压（4kV）和高频（6 kHz）下电离。首先通过使用流式细胞术和细胞周期分析测量经处理的4 T1细胞的细胞活力来研究等离子体处理在杀死癌细胞中的效率。为了探索血浆处理的体内效应，将接种4 T1细胞系的BALB/c小鼠皮下暴露于血浆3分钟。此外，进行H&E染色、TUNEL和Western印迹分析以观察非热等离子体对肿瘤细胞的影响。结果表明，血浆抑制肿瘤生长的效率与典型的化疗药物相当。此外，结果表明，血浆诱导肿瘤组织中的细胞凋亡，并增加凋亡与抗凋亡蛋白表达的比率。我们相信，本文提出的这些发现可能会扩大我们的知识的机制，血浆发挥其有前途的抗癌作用。
This study aimed to evaluate the effects of micron sized non-thermal atmospheric pressure plasma inside the animal body on breast cancer tumor. The μ-plasma jet consists of micron sized hollow tube in which pure helium gas is ionized by high voltage (4 kV) and high frequency (6 kHz). The efficiency of the plasma treatment in killing cancer cells was first investigated by cell viability measurements of treated 4T1 cells using flow cytometry and cell cycle analysis. For exploration of the in vivo effects of the plasma treatment, the BALB/c mice inoculated by 4T1 cell lines were exposed subcutaneously to plasma for 3 minutes. In addition, H&E staining, TUNEL and Western blotting assays were performed in order to observed the effects of the non-thermal plasma on the tumor cells. The results showed that the efficiency of the plasma in suppression of the tumor growth is comparable to that of a typical chemotherapy drug. Moreover, the results indicated that the plasma induces apoptosis in the tumor tissue and increases the ratio of the apoptotic to anti-apoptotic protein expression. We believe that these findings presented herein may extend our knowledge of the mechanisms by which the plasma exerts its promising anti-cancer effects.