Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins
Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins
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DOI：
10.1007/978-1-4939-2205-5_11
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Owens, Raymond J.
中科院分区：
文献类型：
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作者：
Nettleship, Joanne E.;Watson, Peter J.;Owens, Raymond J.
人胚胎肾细胞 (HEK 293) 的瞬时转染能够快速、经济地在实验室规模生产重组蛋白。本章描述了在 HEK 293 细胞中使用瞬时表达来表达和纯化分泌蛋白和细胞内蛋白的方案。
Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.