Novel autologous cell therapy in ischemic limb disease through growth factor secretion by cultured adipose tissue-derived stromal cells
Novel autologous cell therapy in ischemic limb disease through growth factor secretion by cultured adipose tissue-derived stromal cells
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DOI：
10.1161/01.atv.0000190701.92007.6d
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发表时间：
2005-12-01
影响因子：
8.7
通讯作者：
Kaneda, Y
中科院分区：
文献类型：
--
作者：
Nakagami, H;Maeda, K;Kaneda, Y
目的：将自体祖细胞输送到患者的缺血组织中正在成为一种新的治疗选择。在这里，我们报告了培养的脂肪组织来源的细胞（ADSC）对血管生成细胞therapy.Method和Results的潜在影响- ADSC从C57 B1/6小鼠腹股沟脂肪组织中分离，并显示出高表达Sca I和CD 44，但不表达c-kit，Lin，CD 34，CD 45，CD 11b和CD 31，与来自骨髓的间充质干细胞相容。在与ADSC和人主动脉内皮细胞（EC）共培养的条件下，ADSC主要通过分泌血管内皮生长因子（VEGF）和肝细胞生长因子（HGF）来显著增加EC的活力、迁移和管腔形成。ADSC移植于缺血小鼠后肢4周后，应用激光多普勒成像（LDI）和抗CD 31抗体免疫组化法检测ADSC治疗组的血管新生评分。然而，注射的ADSC并不对应于CD 31，血管性血友病因子，和α-平滑肌肌动蛋白阳性cells in ischemic tissue.Conclusion -这些脂肪组织来源的细胞表现出潜在的血管生成细胞治疗缺血性疾病，这似乎主要是通过他们的能力来实现的血管生成生长因子。
Objective - The delivery of autologous progenitor cells into ischemic tissue of patients is emerging as a novel therapeutic option. Here, we report the potential impact of cultured adipose tissue - derived cells (ADSC) on angiogenic cell therapy.Method and Results - ADSC were isolated from C57B1/6 mouse inguinal adipose tissue and showed high expression of ScaI and CD44, but not c-kit, Lin, CD34, CD45, CD11b, and CD31, compatible with that of mesenchymal stem cells from bone marrow. In coculture conditions with ADSC and human aortic endothelial cells (ECs) under treatment with growth factors, ADSC significantly increased EC viability, migration and tube formation mainly through secretion of vascular endothelial growth factor ( VEGF) and hepatocyte growth factor (HGF). At 4 weeks after transplantation of ADSC into the ischemic mouse hindlimb, the angiogenic scores were improved in the ADSC-treated group, which were evaluated with blood flow by laser Doppler imaging (LDI) and capillary density by immunostaining with anti-CD31 antibody. However, injected ADSC did not correspond to CD31, von Willebrand factor, and alpha-smooth muscle actin-positive cells in ischemic tissue.Conclusion - These adipose tissue - derived cells demonstrated potential as angiogenic cell therapy for ischemic disease, which appears to be mainly achieved by their ability to secrete angiogenic growth factors.