Study of rifampin and dapsone resistance in three patients with recurring leprosy
Study of rifampin and dapsone resistance in three patients with recurring leprosy
复制标题
DOI：
10.1590/s1020-49892008000200001
复制
发表时间：
2008-02-01
期刊：
影响因子：
--
通讯作者：
Gómez, Yenny
中科院分区：
文献类型：
--
作者：
Hernández, Elkin;Cardona-Castro, Nora;Gómez, Yenny
Objective.采用分子生物学技术检测3例复发麻风和临床疑似耐药麻风的利福平和氨苯砜耐药株。方法.对哥伦比亚昆迪纳马卡“Agua de Dios”疗养院的3例多菌型麻风病患者进行了回顾性描述性研究，这些患者通过病史、细菌镜检查和活检记录了麻风病复发。对皮肤病变进行活检，并对细菌进行DNA提取和纯化。扩增与抗菌素耐药性相关的rpoB和folP 1基因区域，并进行降落聚合酶链反应，使用桑格法对扩增产物进行测序。结果在两个研究的样本中，在rpoB基因的核苷酸1367中发现了一个点状突变。在这3例患者的folP1基因中均未发现这种突变。结论.突变株为耐利福平的M.三名复发麻风患者中有两名患有麻风病。在三名患者中均未检测到表明对氨苯砜耐药的突变。
Objective. To detect the presence of rifampin- and dapsone- resistant strains of Mycobacterium leprae in three patients with recurring leprosy and clinically- suspected antimicrobial resistance through molecular techniques. Methods. A retrospective, descriptive study was conducted of three multibacillary patients at the " Agua de Dios" Sanitarium in Cundinamarca, Colombia, that presented leprosy relapses that were documented by medical history, bacilloscopy, and biopsy. Biopsies were taken of the skin lesions and the bacteria were subject to DNA extraction and purification. Regions of the rpoB and folP1 genes associated with antimicrobial resistance were amplified and subjected to touch- down polymerase chain reaction and the amplified products were sequenced using the Sanger method. Results. A punctual mutation was identified in nucleotide 1367 of the rpoB gene in two of the samples studied. This mutation was not found in the folP1 gene of any of the three patients. Conclusions. The mutation identified showed strains of rifampin- resistant M. leprae in two of the three patients with recurring leprosy. Mutations that indicate dapsone-resistance were not detected in any of the three patients.