Prevalence and biofilm-forming ability of Listeria monocytogenes in New Zealand mussel (Perna canaliculus) processing plants
Prevalence and biofilm-forming ability of Listeria monocytogenes in New Zealand mussel (Perna canaliculus) processing plants
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DOI：
10.1016/j.fm.2011.06.014
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发表时间：
2011-10-01
影响因子：
5.3
通讯作者：
Fletcher, Graham C.
中科院分区：
文献类型：
--
作者：
Cruz, Cristina D.;Fletcher, Graham C.
绿壳(商标)贻贝是新西兰最大的海鲜出口品种。一些出口市场要求遵守对25克产品中单核细胞增多性李斯特菌的零容忍立法。尽管个别速冻(IQF)贻贝产品被贴上“可烹调”的标签，并未被归类为即食产品，但一些市场仍要求它们遵守严格的政策。对三家贻贝加工厂的原料、环境、食品接触面和成品中L单核细胞增多症的污染模式进行了评估。从2007年8月至2009年6月从工业李斯特氏菌监测项目获得的培养物(n=101)进行了血清分型和脉冲场凝胶电泳法鉴定。使用结晶紫方法，对分离株形成生物膜的能力进行了评估。这项工作证实了生和加工产品中存在单核细胞增多性李斯特菌，以及来自外部和内部环境的交叉污染的重要性。加工厂的L单核细胞增多症脉冲型在6个月内被检测到不止一次。未发现生物被膜形成能力与持久性菌株之间的相关性。两种脉型(包括一种持续性脉型)以前在新西兰的李斯特菌病人病例中分离出来，但没有一种脉型与国际暴发中涉及的脉型相匹配。(C)2011爱思唯尔有限公司。保留所有权利。
Greenshell (TM) mussels are New Zealand's largest seafood export species. Some export markets require compliance with 'zero' tolerance legislation for Listeria monocytogenes in 25 g of product. Even though individually quick frozen (IQF) mussel products are labeled 'to be cooked', and are not classified as ready-to-eat, some markets still require them to comply with the strict policy. Three mussel processing plants were assessed for the pattern of L monocytogenes contamination on raw material, environment, food contact surfaces, and in the final product. Cultures (n = 101) obtained from an industrial Listeria monitoring program from August 2007 to June 2009 were characterized by serotyping and pulsed field gel electrophoresis. Using the crystal violet method, isolates were assessed for their ability to form biofilms. This work confirmed the presence of L. monocytogenes in raw and processed product, and the importance of cross-contamination from external and internal environments. Processing plants had L monocytogenes pulsotypes that were detected more than once over 6 months. No correlation was found between biofilm-forming ability and persistent isolates. Two pulsotypes (including a persistent one), were previously isolated in human cases of listeriosis in New Zealand, but none of the pulsotypes matched those involved in international outbreaks. (C) 2011 Elsevier Ltd. All rights reserved.