Mechanisms of photodynamic inactivation of herpes simplex viruses: comparison between methylene blue, light plus electricity, and hematoporhyrin plus light.
Mechanisms of photodynamic inactivation of herpes simplex viruses: comparison between methylene blue, light plus electricity, and hematoporhyrin plus light.
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单纯疱疹病毒光动力灭活机制：亚甲蓝、光加电和血卟啉加光的比较。
DOI：
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发表时间：
1980
影响因子：
15.9
通讯作者：
C. Crumpacker
中科院分区：
文献类型：
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作者：
L. Schnipper;A. Lewin;M. Swartz;C. Crumpacker
单纯疱疹病毒(HSV)1型和2型在体外用低浓度的亚甲蓝(MB)、光(Lambda)加电(E)或血卟啉衍生物(Hpd)加lambda灭活。这两种技术都将单链中断引入病毒DNA，但不会使病毒DNA双链断裂，但不会造成双链断裂。MB、lambda加E处理的病毒粒子能正常吸附和穿透敏感细胞，而HSV用HPC和LIGH灭活不能。这种差异在感染MB、lambda加E处理的病毒粒子后诱导新的病毒和细胞DNA合成方面得到了强调，而在HPD和lambda暴露后只产生了细胞DNA，而没有HSV DNA。这些观察结果反映了病毒失活的不同机制。在病毒DNA合成后，病毒成熟受阻(S)，这是MB、lambda和E处理的结果，而Hpd加上Lambda处理的颗粒无法进入敏感细胞，因此不会引发感染。
Herpes simplex virus (HSV) types 1 and 2 have been inactivated in vitro using low concentrations of methylene blue (MB), light (lambda) plus electricity (E), or hematoporphyrin derivative (HPD) plus lambda. Both techniques introduce single strand interruptions into viral DNA, but do not make double strand ruptions into viral DNA, but do not make double strand breaks. MB, lambda plus E-treated virions adsorb normally to and penetrate susceptible cells, whereas HSV inactivated with HPC and light does not. This difference is emphasized by the induction of new viral and cell DNA synthesis after infection with MB, lambda plus E-treated virions, whereas only cell, DNA but no HSV DNA, is made subsequent to HPD and lambda exposure. These observations reflect disparate mechanisms of viral inactivation. A block(s) in viral maturation, subsequent to viral DNA synthesis, occurs as a result of treatment with MB, lambda and E, whereas HPD plus lambda-treated particles fail to enter a susceptible cell, and therefore do not initiate an infection.