The optical stretcher:: A novel laser tool to micromanipulate cells
The optical stretcher:: A novel laser tool to micromanipulate cells
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DOI：
10.1016/s0006-3495(01)75740-2
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发表时间：
2001-08-01
影响因子：
3.4
通讯作者：
Käs, J
中科院分区：
文献类型：
--
作者：
Guck, J;Ananthakrishnan, R;Käs, J
当一个介电物体被放置在两个相对的、非聚焦的激光光束之间时，作用在该物体上的总力为零，但表面力是附加的，从而导致物体沿光束的轴向拉伸。利用这一原理，我们已经构建了一种名为光学拉伸器的设备，它可以用于测量包括细胞等生物材料在内的介电材料的粘弹性属性，具有必要的灵敏度，甚至可以区分不同的单个细胞骨架表型。我们已经成功地使用光学拉伸器使人红细胞和小鼠成纤维细胞变形。在光学展宽器中，不需要聚焦，因此辐射损伤最小，表面力不受光功率的限制。光学拉伸器中的变形力的大小因此为研究生物材料的光学镊子和原子力显微镜之间架起了一座桥梁。
When a dielectric object is placed between two opposed, nonfocused laser beams, the total force acting on the object is zero but the surface forces are additive, thus leading to a stretching of the object along the axis of the beams. Using this principle, we have constructed a device, called an optical stretcher, that can be used to measure the viscoelastic properties of dielectric materials, including biologic materials such as cells, with the sensitivity necessary to distinguish even between different individual cytoskeletal phenotypes. We have successfully used the optical stretcher to deform human erythrocytes and mouse fibroblasts. In the optical stretcher, no focusing is required, thus radiation damage is minimized and the surface forces are not limited by the light power. The magnitude of the deforming forces in the optical stretcher thus bridges the gap between optical tweezers and atomic force microscopy for the study of biologic materials.