CHARACTERIZATION AND DISTRIBUTION OF THE HEMAGGLUTININS PRODUCED BY VIBRIO-CHOLERAE
CHARACTERIZATION AND DISTRIBUTION OF THE HEMAGGLUTININS PRODUCED BY VIBRIO-CHOLERAE
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DOI：
10.1128/iai.36.1.209-214.1982
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
FINKELSTEIN, RA
中科院分区：
文献类型：
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作者：
HANNE, LF;FINKELSTEIN, RA
对霍乱弧菌产生的细胞相关和可溶性血凝素（HA）的分布进行了检查，发现存在4种不同的HA。一个细胞相关的甘露糖敏感的HA（MSHA）只产生的El Tor生物型。这在所有检查的El Tor菌株中是明显的。它似乎负责El Tor与经典生物型霍乱弧菌的HA生物分型区分。MSHA没有明显的二价离子需求;它被D-甘露糖和D-果糖抑制;它对所有人（A、B、O）和所有鸡红细胞都有活性。通过MSHA+亲本细菌与红细胞的共沉降来选择El Tor菌株的自发MSHA-突变体。L-岩藻糖敏感性HA在所检查的4种经典菌株中的2种和El Tor生物型菌株3083、26-3和17的MSHA突变体的早期生长中被瞬时检测到。MSHA突变体还在后期对数期培养物中表达另一种细胞相关HA。在所有测试菌株的后期对数期培养物中检测到可溶性HA。该HA不能被任何测试的糖替代;它需要CA 2+离子以获得最大活性;它对一些鸡红细胞有活性，但对其他人没有活性。
Examination of the distribution of cell-associated and soluble hemagglutinins (HA) produced by V. cholerae revealed the existance of 4 different HA. A cell-associated mannose-sensitive HA (MSHA) was produced only by the El Tor biotype. This was evident with all El Tor strains examined. It appears to be responsible for the HA biotyping differentiation of El Tor from classical biotype V. cholerae. The MSHA had no apparent divalent ion requirement; it was inhibited by D-mannose and D-fructose; it was active on all human (A, B, O) and all chicken erythrocytes tested. Spontaneous MSHA- mutants of El Tor strains were selected by cosedimentation of MSHA+ parent bacteria with erythrocytes. An L-fucose-sensitive HA was detected transiently in early lophase growth with 2 of the 4 classical strains examined and with MSHA- mutants of El Tor biotype strains 3083, 26-3 and 17. MSHA- mutants also expressed another cell-associated HA in late log-phase cultures. A soluble HA was detected in late log-phase cultures of all strains tested. This HA was not inhibitable by any sugars tested; it required CA2+ ions for maximum activity; it was active on some chicken erythrocytes but not others.