An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells.
An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells.
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DOI：
10.1093/jxb/ern280
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发表时间：
2009
影响因子：
6.9
通讯作者：
Shimmen T
中科院分区：
文献类型：
--
作者：
Yokota E;Ueda S;Tamura K;Orii H;Uchi S;Sonobe S;Hara-Nishimura I;Shimmen T
肌球蛋白XI参与产生植物细胞中细胞质流动的动力变得越来越明显。为了全面了解肌球蛋白XI异构体的生理作用，有必要单独阐明每个异构体的性质和功能。在烟草培养的BY-2细胞中，已经通过生物化学和免疫细胞化学鉴定了两种类型的肌球蛋白，一种由175 kDa重链（175 kDa肌球蛋白）组成，另一种由170 kDa重链（170 kDa肌球蛋白）组成。根据编码175 kDa和170 kDa肌球蛋白重链的cDNA克隆的序列分析，这两种肌球蛋白均被归类为肌球蛋白XI。使用纯化的175 kDa肌球蛋白重链的多克隆抗体的免疫细胞化学研究表明，175 kDa肌球蛋白分布在整个细胞质中的间期BY-2细胞的细点。在有丝分裂过程中，175 kDa肌球蛋白的一部分被发现积累在前前期带（PPB），纺锤体，赤道平面的成膜体和周围的子核。在转基因BY-2细胞中，其中内质网（ER）特异性滞留信号，HDEL，标记有绿色荧光蛋白（GFP）稳定表达，ER表现出类似的行为，175 kDa肌球蛋白。此外，该肌球蛋白与GFP-ER共分级通过蔗糖密度梯度离心。从这些发现，这表明，175 kDa肌球蛋白是一个分子马达负责易位ER在BY-2细胞。
The involvement of myosin XI in generating the motive force for cytoplasmic streaming in plant cells is becoming evident. For a comprehensive understanding of the physiological roles of myosin XI isoforms, it is necessary to elucidate the properties and functions of each isoform individually. In tobacco cultured BY-2 cells, two types of myosins, one composed of 175 kDa heavy chain (175 kDa myosin) and the other of 170 kDa heavy chain (170 kDa myosin), have been identified biochemically and immunocytochemically. From sequence analyses of cDNA clones encoding heavy chains of 175 kDa and 170 kDa myosin, both myosins have been classified as myosin XI. Immunocytochemical studies using a polyclonal antibody against purified 175 kDa myosin heavy chain showed that the 175 kDa myosin is distributed throughout the cytoplasm as fine dots in interphase BY-2 cells. During mitosis, some parts of 175 kDa myosin were found to accumulate in the pre-prophase band (PPB), spindle, the equatorial plane of a phragmoplast and on the circumference of daughter nuclei. In transgenic BY-2 cells, in which an endoplasmic reticulum (ER)-specific retention signal, HDEL, tagged with green fluorescent protein (GFP) was stably expressed, ER showed a similar behaviour to that of 175 kDa myosin. Furthermore, this myosin was co-fractionated with GFP–ER by sucrose density gradient centrifugation. From these findings, it was suggested that the 175 kDa myosin is a molecular motor responsible for translocating ER in BY-2 cells.
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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影响因子：
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作者：
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