Expression Analysis of Restorer Alleles-Induced Genes in Pepper
Expression Analysis of Restorer Alleles-Induced Genes in Pepper
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DOI：
10.1016/s1671-2927(11)60088-6
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发表时间：
2011-07-20
影响因子：
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通讯作者：
Shen Huo-lin
中科院分区：
文献类型：
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作者：
Guo Shuang;Ma Ning;Shen Huo-lin
辣椒（Capsicum annuum L.）细胞质雄性不育的育性恢复对于杂交种子的商业生产很有用。然而，生育力恢复的机制尚未确定。我们之前构建了 cDNA 文库，并利用抑制消减杂交技术鉴定了一些与辣椒育性恢复相关的基因。在本研究中，使用半定量 RT-PCR 研究了 20 个基因的表达模式。三个基因仅在恢复系中表达，但在不育系中不表达。四个基因仅在花药中表达，而在其他器官中不表达。在这7个基因中，观察到克隆TG31在恢复系的花药中特异性表达。这里描述的工作提供了对辣椒恢复等位基因诱导的基因表达模式的全面概述。它还将有助于当前对调节生育力恢复的分子网络的理解。
Fertility restoration of cytoplasmic male-sterility in pepper (Capsicum annuum L.) is useful for commercial production of hybrid seeds. However, the mechanism of fertility restoration has not been determined. We previously constructed a cDNA library and identified some genes related to fertility restoration in pepper using suppression subtractive hybridization technology. In this study, the expression patterns of 20 genes were investigated using semi-quantitative RT-PCR. Three genes expressed only in restorer lines, but not in sterility lines. Four genes expressed only in anther, but not in other organs. Among these 7 genes, the clone TG31 was observed to specifically express in anther of restorer lines. The work described here provides a comprehensive overview on the expression pattern of the genes that are induced by restorer alleles in pepper. It will also contribute to the current understanding of molecular networks for the regulation of fertility restoration.