Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats.
Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats.
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DOI：
10.1155/2015/390428
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发表时间：
2015
影响因子：
4.3
通讯作者：
Chen LM
中科院分区：
文献类型：
--
作者：
Ma ZJ;Zhang XN;Li L;Yang W;Wang SS;Guo X;Sun P;Chen LM
雷公藤多甙片（TGT）是一种中药，已被证明可以保护足细胞免受损伤并减少蛋白尿。本研究的目的是评估 TGT 对高脂饮食喂养和 STZ 诱导的糖尿病大鼠肾小管间质纤维化的影响及其潜在机制。将大鼠随机分为正常对照大鼠（NC组）、未药物治疗的糖尿病大鼠（DM组）和接受TGT（分别为1、3或6mg/kg/天）治疗8周的糖尿病大鼠。结果显示，TGT治疗可降低糖尿病大鼠24小时蛋白尿和尿N-乙酰氨基葡萄糖苷酶(NAG)，且不影响血糖。 Masson三色染色显示DM组肾小管间质纤维化明显，经TGT治疗后可改善。在 TGT 治疗的大鼠中，α-SMA 的表达显着降低，同时 E-钙粘蛋白的表达增加，但在未治疗的 DM 大鼠中则不然。进一步的研究表明，TGT 治疗的糖尿病大鼠中 TLR4、NF-κB、IL-1β 和 MCP-1 的表达显着降低。这些结果表明TGT可以改善肾小管间质纤维化，该机制可能至少部分与通过抑制TLR4/NF-κB通路改善EMT有关。
Tripterygium glycosides tablet (TGT) is a Chinese traditional medicine that has been shown to protect podocytes from injury and reduce the proteinuria. The aim of this study was to assess the effect of TGT on renal tubulointerstitial fibrosis and its potential mechanism in high-fat diet fed and STZ-induced diabetic rats. Rats were randomly divided into normal control rats (NC group), diabetic rats without drug treatment (DM group), and diabetic rats treated with TGT (1, 3, or 6 mg/kg/day, respectively) for 8 weeks. The results showed that 24 h proteinuria and urinary N-acetyl-glucosaminidase (NAG) in diabetic rats were decreased by TGT treatment without affecting blood glucose. Masson's trichrome stains showed that apparent renal tubulointerstitial fibrosis was found in DM group, which was ameliorated by TGT treatment. The expression of α-SMA was significantly decreased, accompanied by increased expression of E-cadherin in TGT-treated rats, but not in untreated DM rats. Further studies showed that TGT administration markedly reduced expression of TLR4, NF-κB, IL-1β, and MCP-1 in TGT-treated diabetic rats. These results showed that TGT could ameliorate renal tubulointerstitial fibrosis, the mechanism which may be at least partly associated with the amelioration of EMT through suppression of the TLR4/NF-κB pathway.