Illuminating the cells: transient transformation of citrus to study gene functions and organelle activities related to fruit quality.
Illuminating the cells: transient transformation of citrus to study gene functions and organelle activities related to fruit quality.
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照亮细胞：柑橘的瞬时转化以研究与水果品质相关的基因功能和细胞器活动
DOI：
10.1038/s41438-021-00611-1
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发表时间：
2021-08-01
影响因子：
8.7
通讯作者：
Wang P
中科院分区：
文献类型：
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作者：
Gong J;Tian Z;Qu X;Meng Q;Guan Y;Liu P;Chen C;Deng X;Guo W;Cheng Y;Wang P
虽然多种显微技术已被应用于园艺研究，很少有研究活的水果细胞中的单个细胞器的日期已被报道。本研究利用农杆菌介导法建立了柑橘瞬时转化体系。使用金桔（金桔）;它表现出比所有已测试的柑橘类水果更高的转化效率和延长的表达窗口。用荧光报告基因转化果实，并使用共聚焦显微镜和活细胞成像来研究它们的定位和动力学。此外，表达了针对不同亚细胞区室的各种pH传感器，并比较了来自不同植物组织的细胞中的局部pH环境。结果表明，液泡可能是导致柑橘果实pH值偏低的主要细胞器。总之，我们的方法是有效的研究各种膜运输事件，蛋白质定位和细胞生理在水果，可以提供新的见解水果生物学研究。
Although multiple microscopic techniques have been applied to horticultural research, few studies of individual organelles in living fruit cells have been reported to date. In this paper, we established an efficient system for the transient transformation of citrus fruits using an Agrobacterium-mediated method. Kumquat (Fortunella crassifolia Swingle) was used; it exhibits higher transformation efficiency than all citrus fruits that have been tested and a prolonged-expression window. Fruits were transformed with fluorescent reporters, and confocal microscopy and live-cell imaging were used to study their localization and dynamics. Moreover, various pH sensors targeting different subcellular compartments were expressed, and the local pH environments in cells from different plant tissues were compared. The results indicated that vacuoles are most likely the main organelles that contribute to the low pH of citrus fruits. In summary, our method is effective for studying various membrane trafficking events, protein localization, and cell physiology in fruit and can provide new insight into fruit biology research.
DOI：
10.3390/s18041163
发表时间：
2018-04-11
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Hu Y;Yang D;Yang C;Feng N;Shao Z;Zhang L;Wang X;Weng L;Luo Z;Wang L
通讯作者：
Wang L