EFFECTS OF CHOLECYSTOKININ AND CARBAMYLCHOLINE ON PANETH CELL SECRETION IN MICE - A COMPARISON WITH PANCREATIC ACINAR-CELLS
EFFECTS OF CHOLECYSTOKININ AND CARBAMYLCHOLINE ON PANETH CELL SECRETION IN MICE - A COMPARISON WITH PANCREATIC ACINAR-CELLS
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DOI：
10.1002/ar.1092250207
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发表时间：
1989-10-01
影响因子：
--
通讯作者：
ONO, K
中科院分区：
文献类型：
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作者：
SATOH, Y;ISHIKAWA, K;ONO, K
为了证实小鼠（ICR，雄性，10-12周龄）的潘氏细胞是否与胰腺腺泡细胞一样对激素和胆碱能刺激具有相同的分泌反应，在给予不同剂量的胆囊收缩素（八肽，CCK-8）和氨甲酰胆碱后1小时，对潘氏细胞和胰腺腺泡细胞的超微结构变化进行了形态测定。在最大剂量（1.5 μ g/kg腹膜内[i. p.]）和超最大剂量（15 μ g/kg，i. p.）在CCK-8的刺激下，胰腺腺泡细胞分别表现出脱粒或分泌紊乱（例如，溶酶体样小体增加，酶原颗粒聚集）。然而，潘氏细胞的参数几乎没有变化。注射氨甲酰胆碱（1，000 μ g/kg，皮下[s.c.]）后，胰腺腺泡细胞和潘氏细胞均显示脱颗粒。潘氏细胞有时会出现大空泡，可能是在大量胞吐后形成的;胰腺腺泡细胞中未观察到此类空泡。提示潘氏细胞和胰腺腺泡细胞具有不同的分泌反应。潘氏细胞分泌可能在控制肠道细菌环境中起作用，可能是由胆碱能机制而不是激素机制刺激的。
To confirm whether the Paneth cells of mice (ICR, male, 10-12 weeks old) have the same secretory response to hormonal and cholinergic stimulation as do pancreatic acinar cells, ultrastructural changes of Paneth cells and pancreatic acinar cells 1 hr after administration of various doses of cholecystokinin (octapeptide, CCK-8) and carbamylcholine were morphometrically assessed. After maximal (1.5 .mu.g/kg intraperitoneally [i.p.]) and supramaximal (15 .mu.g/kg, i.p.) stimulation by CCK-8, pancreatic acinar cells showed, respectively, degranulation or disturbances of secretion (e.g., an increase in lysosome-like bodies, aggregation of zymogen granules). The Paneth cells, however, were almost unchanged in the parameters examined. After carbamylcholine injection (1,000 .mu.g/kg, subcutaneously [s.c.]), both pancreatic acinar cells and Paneth cells showed degranulation. Paneth cells sometimes developed large vacuoles, probably formed after massive exocytosis; such vacuoles were not observed in pancreatic acinar cells. It is suggested that Paneth cells and pancreatic acinar cells have different secretory responses. Paneth cell secretion, whcih possibly plays a role in controlling the intestinal bacterial milieu, may be stimulated by cholinergic rather thanhormonal mechanisms.