Alterations in the biosynthesis of lignin in transgenic plants with chimeric genes for 4-coumarate:coenzyme a ligase
Alterations in the biosynthesis of lignin in transgenic plants with chimeric genes for 4-coumarate:coenzyme a ligase
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DOI：
10.1093/oxfordjournals.pcp.a029045
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发表时间：
1996-10-01
影响因子：
4.9
通讯作者：
Omori, S
中科院分区：
文献类型：
--
作者：
Kajita, S;Katayama, Y;Omori, S
将4-香豆酸：辅酶A连接酶的正、反义嵌合基因导入烟草植株后，转基因植株的4CL活性降低。转基因植株茎中木质部组织的细胞壁呈棕色，有色细胞壁中木质素的分子结构与对照植株有显著差异。不同类型着色剂的分析表明，木质素中的肉桂醛残留量和丁香基单位在棕色的细胞壁中被抑制。此外，有色组织中的木质素含量低于正常组织。我们的结果表明，4CL在测定烟草中木质素的组成和含量方面具有重要作用。
The introduction of chimeric sense and antisense gene constructs for 4-coumarate:coenzyme A ligase into tobacco plants caused the reduction of the 4CL activity in the transgenic plants. In the transgenic plants, the cell walls of the xylem tissue in stems were brown and the molecular structure of lignin in the colored cell walls was dramatically different from that in the control plants. Analysis with different types of stain revealed that levels of cinnamyl aldehyde residues and syringyl units in lignin were depressed in the brownish cell walls. Furthermore, the lignin content in colored tissue was lower than that in the normal tissue. Our results indicate that 4CL has important roles in the determination of the composition and the amount of lignin in tobacco plants.